Rabbit JNK3 Polyclonal Antibody | anti-JNK3 antibody

JNK3 (K391) Polyclonal Antibody

Cat. Num. AAA3002051

• Gene Names: MAPK10; JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: JNK3; Polyclonal Antibody; JNK3 (K391) Polyclonal Antibody; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; MAPK10; JNK3A; PRKM10; SAPK1B; anti-JNK3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: JNK3 (K391) polyclonal antibody detects endogenous levels of JNK3 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 422

• Applicable Applications for anti-JNK3 antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; IF: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 380-420 of Human JNK3.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of JNK3 (K391) pAb at 1:1000 dilutionLane1:C6 whole cell lysate (40ug) Lane2:CT26 whole cell lysate (40ug) Lane3:U-87MG whole cell lysate (40ug) Lane4:MCF-7 whole cell lysate (40ug))

Immunohistochemistry (IHC)

(Immunohistochemistry (IHC) analyzes of JNK3 (K391) pAb in paraffin-embedded human brain tissue.)

Western Blot (WB)

(Western Blot (WB) analysis of JNK3 (K391) pAb at 1:500 dillutionLane1:Hela whole cell lysate (30ug) Lane2:NIH-3T3 whole cell lysate (30ug) Lane3:PC12 whole cell lysate (30ug))

Related Product Information for anti-JNK3 antibody

MAPK10 (JNK3) is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This protein is a neuron-specific form of c-Jun N-terminal kinases (JNKs). Through its phosphorylation and nuclear localization, this kinase plays regulatory roles in the signaling pathways of neuronal apoptosis. Beta-arrestin 2, a receptor-regulated MAP kinase scaffold protein, is found to interact with and stimulate the phosphorylation of this kinase by MAP kinase kinase 4 (MKK4). Cyclin-dependent kianse 5 (CDK5) can phosphorylate and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Four alternatively spliced transcript variants encoding distinct isoforms have been reported.

NCBI and Uniprot Product Information

• NCBI GI #: 4506081
• NCBI GeneID: 5602
• NCBI Accession #: NP_002744.1
• NCBI GenBank Nucleotide #: NP_002744.1
• UniProt Accession #: P53779
• Molecular Weight: ~ 53kDa
• NCBI Official Full Name: mitogen-activated protein kinase 10 isoform 2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 10
• NCBI Official Symbol: MAPK10
• NCBI Official Synonym Symbols: JNK3; JNK3A; PRKM10; SAPK1b; p493F12; p54bSAPK
• NCBI Protein Information: mitogen-activated protein kinase 10
• UniProt Protein Name: Mitogen-activated protein kinase 10
• UniProt Gene Name: MAPK10
• UniProt Synonym Gene Names: JNK3; JNK3A; PRKM10; SAPK1B; MAP kinase 10; MAPK 10; SAPK1b
• UniProt Entry Name: MK10_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as integration points for multiple biochemical signals, and thus are involved in a wide variety of cellular processes, such as proliferation, differentiation, transcription regulation and development. This kinase is specifically expressed in a subset of neurons in the nervous system, and is activated by threonine and tyrosine phosphorylation. Targeted deletion of this gene in mice suggests that it may have a role in stress-induced neuronal apoptosis. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. A recent study provided evidence for translational readthrough in this gene, and expression of an additional C-terminally extended isoform via the use of an alternative in-frame translation termination codon. [provided by RefSeq, Dec 2017]

Uniprot Description

JNK3: a protein kinase of the MAPK family that is potently activated by a variety of environmental stress and pro-inflammatory cytokines. Brain-selective JNK isoform. A pro-apoptotic protein and potential tumor suppressor. Phosphorylates a number of transcription factors including c-Jun, ATF2 and ELK1. Required for stress-induced neuronal apoptosis and the pathogenesis of glutamate excitotoxicity. CDK5 can phosphorylate and inhibit the activity of this kinase, which may be important in preventing neuronal apoptosis. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Interacts with HDAC9. Expression lost in brain tumors. May function in neuronal cell death from injury and neurodegeneration, for which inhibitors are being developed. Two alternatively spliced transcript variants have been reported.

Protein type: Protein kinase, CMGC; Kinase, protein; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily

Chromosomal Location of Human Ortholog: 4q22.1-q23

Cellular Component: nucleoplasm; mitochondrion; cytoplasm; plasma membrane; cytosol

Molecular Function: MAP kinase kinase activity; protein binding; JUN kinase activity; ATP binding

Biological Process: response to light stimulus; activation of MAPK activity; MyD88-independent toll-like receptor signaling pathway; stress-activated MAPK cascade; rhythmic process; toll-like receptor 3 signaling pathway; regulation of circadian rhythm; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; JUN phosphorylation; toll-like receptor 5 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; regulation of transcription factor activity; toll-like receptor signaling pathway; JNK cascade; innate immune response; toll-like receptor 9 signaling pathway; toll-like receptor 4 signaling pathway