Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit | vHL elisa kit

Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit

Cat. Num. AAA2706761

• Gene Names: VHL; RCA1; VHL1; pVHL; HRCA1
• Reactivity: Rat
• Synonyms: Von Hippel Lindau Tumor Suppressor (vHL); Rat Von Hippel Lindau Tumor Suppressor (vHL) ELISA Kit; HRCA1; RCA1; VHL1; Von Hippel-Lindau Syndrome; vHL elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Von Hippel Lindau Tumor Suppressor (vHL).; No significant cross-reactivity or interference between Von Hippel Lindau Tumor Suppressor (vHL) and analogues was observed.
• Sequence Length: 160

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Von Hippel Lindau Tumor Suppressor (vHL) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Von Hippel Lindau Tumor Suppressor (vHL) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for vHL elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Von Hippel Lindau Tumor Suppressor (vHL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Von Hippel Lindau Tumor Suppressor (vHL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Von Hippel Lindau Tumor Suppressor (vHL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Von Hippel Lindau Tumor Suppressor (vHL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 38045906
• NCBI GeneID: 7428
• NCBI Accession #: NP_937799.1
• NCBI GenBank Nucleotide #: NM_198156.2
• UniProt Accession #: P40337; Q13599; Q6PDA9; B2RE45
• Molecular Weight: 18,532 Da
• NCBI Official Full Name: von Hippel-Lindau disease tumor suppressor isoform 2
• NCBI Official Synonym Full Names: von Hippel-Lindau tumor suppressor
• NCBI Official Symbol: VHL
• NCBI Official Synonym Symbols: RCA1; VHL1; pVHL; HRCA1
• NCBI Protein Information: von Hippel-Lindau disease tumor suppressor
• UniProt Protein Name: von Hippel-Lindau disease tumor suppressor
• Protein Family: Von Hippel-Lindau disease tumor suppressor
• UniProt Gene Name: VHL

NCBI Description

Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of this gene is the basis of familial inheritance of VHL syndrome. The protein encoded by this gene is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in the ubiquitination and subsequent proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Seems to act as a target recruitment subunit in the E3 ubiquitin ligase complex and recruits hydroxylated hypoxia-inducible factor (HIF) under normoxic conditions. Involved in transcriptional repression through interaction with HIF1A, HIF1AN and histone deacetylases. Ubiquitinates, in an oxygen-responsive manner, ADRB2.