Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using JNK1/JNK2 (Phospho-Thr183/Tyr185) antibody)
Rabbit JNK1/JNK2 Polyclonal Antibody | anti-JNK1/JNK2 antibody
JNK1/JNK2 antibody
Applications
Immunofluorescence, Western Blot
Purity
JNK1/JNK2 antibody was purified by immunoaffinity chromatography.
Synonyms
JNK1/JNK2; Polyclonal Antibody; JNK1/JNK2 antibody; Polyclonal JNK1/JNK2; Anti-JNK1/JNK2; JNK/JNK2-1; Phospho Thr183/Tyr185; JNK/JNK2 1; c-Jun N-terminal kinase; anti-JNK1/JNK2 antibody
Host
Rabbit
Clonality
Polyclonal
Purity/Purification
JNK1/JNK2 antibody was purified by immunoaffinity chromatography.
Form/Format
Liquid in PBS, pH 7.4, with 150mM NaCl, 0.02% Sodium Azide, and 50% Glycerol
Concentration
1.0 mg/ml (varies by lot)
Applicable Applications for anti-JNK1/JNK2 antibody
Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 1:100-1:200
WB: 1:500-1:1000
WB: 1:500-1:1000
Biological Significance
c-Jun N-terminal kinases (JNKs), were originally identified as kinases that bind and phosphorylate c-Jun on Ser-63 and Ser-73 within its transcriptional activation domain. They belong to the mitogen-activated protein kinase family, and are responsive to stress stimuli, such as cytokines, ultraviolet irradiation, heat shock, and osmotic shock. They also play a role in T cell differentiation and the cellular apoptosis pathway. Activation occurs through a dual phosphorylation of threonine (Thr) and tyrosine (Tyr) residues within a Thr-Pro-Tyr motif located in kinase subdomain VIII.
Cross-Reactivity
Human, Mouse, Rat
Immunogen
Peptide sequence around phosphorylation site of Thr183/Tyr185 derived from Human JMK1/JNK2/JNK3.
Preparation and Storage
Store at 4 degree C short term; Store at -20 degree C long term
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using JNK1/JNK2 (Phospho-Thr183/Tyr185) antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of methanol-fixed Hela cells using JNK1/JNK2 (Phospho-Thr183/Tyr185) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from C6 cells untreated or treated with anisomycin using JNK1/JNK2 (Phospho-Thr183/Tyr185) antibody)
Western Blot (WB)
(Western Blot analysis of extracts from C6 cells untreated or treated with anisomycin using JNK1/JNK2 (Phospho-Thr183/Tyr185) antibody)
Related Product Information for anti-JNK1/JNK2 antibody
Rabbit polyclonal JNK1/JNK2 antibody
Product Categories/Family for anti-JNK1/JNK2 antibody
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Product Notes
The JNK1/JNK2 (Catalog #AAA835724) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's JNK1/JNK2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescence (IF), Western Blot (WB). IF: 1:100-1:200 WB: 1:500-1:1000. Researchers should empirically determine the suitability of the JNK1/JNK2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK1/JNK2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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