Rabbit JNK1/2/3 Polyclonal Antibody | anti-MAPK8 antibody

Anti-JNK1/2/3 (pT183/Y185) Phospho Antibody

Cat. Num. AAA8241791

• Gene Names: MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• Reactivity: Human, Mouse, Rat, Chicken
• Applications: Western Blot
• Purity: The antibody was purified by immunogen affinity chromatography.
• Synonyms: JNK1/2/3; Polyclonal Antibody; Anti-JNK1/2/3 (pT183/Y185) Phospho Antibody; MAPK8; JNK1; PRKM8; SAPK1; SAPK1C; Mitogen-activated protein kinase 8; MAP kinase 8; MAPK 8; JNK-46; Stress-activated protein kinase 1c; SAPK1c; Stress-activated protein kinase JNK1; c-Jun N-terminal kinase 1; MAPK9; JNK2; PRKM9; SAPK1A; Mitogen-activated protein kinase 9; MAP kinase 9; MAPK 9; JNK-55; Stress-activated protein kinase 1a; SAPK1a; Stress-activated protein kinase JNK2; c-Jun N-terminal kinase 2; MAPK10; JNK3; JNK3A; PRKM10; SAPK1B; Mitogen-activated protein kinase 10; MAP kinase 10; MAPK 10; MAP kinase p49 3F12; Stress-activated protein kinase 1b; SAPK1b; Stress-activated protein kinase JNK3; c-Jun N-terminal kinase 3; anti-MAPK8 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Chicken
• Clonality: Polyclonal
• Specificity: Recognizes endogenous levels of JNK1/2/3 (pT183/Y185) protein.
• Purity/Purification: The antibody was purified by immunogen affinity chromatography.
• Form/Format: Liquid in 0.42% Potassium Phosphate, 0.87% Sodium Chloride, pH 7.3, 30% Glycerol, and 0.01% Sodium Azide.
• Sequence Length: 427

• Applicable Applications for anti-MAPK8 antibody: Western Blot (WB)
• Application Notes: Western Blot: (1/500 - 1/1000)
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human JNK1/2/3. The exact sequence is proprietary.
• Preparation and Storage: Shipped and store at 4 degree C for one year. Do not freeze.

Western Blot (WB)

(Western blot analysis of JNK1/2/3 (pT183/Y185) expression in MCF7 UV-treated (A), SP20 UV-treated (B), PC12 UV-treated (C) whole cell lysates.)

Related Product Information for anti-MAPK8 antibody

Rabbit polyclonal antibody to JNK1/2/3 (pT183/Y185)

NCBI and Uniprot Product Information

• NCBI GI #: 2507195
• NCBI GeneID: 5599
• UniProt Accession #: P45983; Q15709; Q15712; Q15713; Q308M2; B5BTZ5; B7ZLV4; D3DX88; D3DX92
• Molecular Weight: 35,333 Da
• NCBI Official Full Name: Mitogen-activated protein kinase 8
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: MAPK8
• NCBI Official Synonym Symbols: JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• NCBI Protein Information: mitogen-activated protein kinase 8
• UniProt Protein Name: Mitogen-activated protein kinase 8
• UniProt Gene Name: MAPK8
• UniProt Synonym Gene Names: JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c
• UniProt Entry Name: MK08_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Several alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Apr 2016]

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: EC 2.7.11.24; Protein kinase, CMGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: cytosol; mitochondrion; nucleoplasm; nucleus; podosome

Molecular Function: ATP binding; enzyme binding; histone deacetylase binding; histone deacetylase regulator activity; JUN kinase activity; protein binding; protein serine/threonine kinase activity

Biological Process: apoptosis; DNA repair; double-strand break repair; innate immune response; JNK cascade; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; MyD88-independent toll-like receptor signaling pathway; negative regulation of apoptosis; negative regulation of protein binding; nerve growth factor receptor signaling pathway; ossification; peptidyl-serine phosphorylation; peptidyl-threonine phosphorylation; positive regulation of apoptosis; positive regulation of cyclase activity; positive regulation of protein metabolic process; programmed cell death; protein amino acid phosphorylation; regulation of circadian rhythm; regulation of histone deacetylation; regulation of protein localization; regulation of transcription factor activity; response to cadmium ion; response to stress; response to UV; rhythmic process; stress-activated MAPK cascade; toll-like receptor 10 signaling pathway; toll-like receptor 2 signaling pathway; toll-like receptor 3 signaling pathway; toll-like receptor 4 signaling pathway; toll-like receptor 5 signaling pathway; toll-like receptor 9 signaling pathway; toll-like receptor signaling pathway