Western Blot (WB)
(Western blot analysis of JNK1/2/3 (pT183) expression in MCF7 PMA-treated (A), NIH3T3 UV-treated (B), PC12 PMA-treated (C) whole cell lysates.)
Rabbit JNK1/2/3 (pT183) Polyclonal Antibody | anti-MAPK8 antibody
Anti-JNK1/2/3 (pT183) Phospho Antibody
IH (1/100 - 1/200)
IF/IC (1/100 - 1/500)
26419, 26420 (Mouse)
116554,50658, 25272 (Rat)
Q91Y86, Q9WTU6, Q61831 (Mouse)
P49185, P49186, P49187 (Rat)
Western Blot (WB)
(Western blot analysis of JNK1/2/3 (pT183) expression in MCF7 PMA-treated (A), NIH3T3 UV-treated (B), PC12 PMA-treated (C) whole cell lysates.)
Western Blot (WB)
(Western blot analysis of JNK1/2/3 (pT183) expression in MCF7 PMA-treated (A), NIH3T3 UV-treated (B), PC12 PMA-treated (C) whole cell lysates.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of JNK1/2/3 (pT183) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunohistochemistry (IHC)
(Immunohistochemical analysis of JNK1/2/3 (pT183) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
Immunofluorescence (IF)
(Immunofluorescent analysis of JNK1/2/3 (pT183) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
Immunofluorescence (IF)
(Immunofluorescent analysis of JNK1/2/3 (pT183) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]
Uniprot Description
JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.
Protein type: Kinase, protein; Protein kinase, CMGC; EC 2.7.11.24; Protein kinase, Ser/Thr (non-receptor); CMGC group; MAPK family; JNK subfamily; MAPK/JNK subfamily
Chromosomal Location of Human Ortholog: 10q11.22
Cellular Component: nucleoplasm; mitochondrion; cytosol; nucleus
Molecular Function: protein serine/threonine kinase activity; protein binding; histone deacetylase regulator activity; enzyme binding; histone deacetylase binding; JUN kinase activity; ATP binding
Biological Process: nerve growth factor receptor signaling pathway; apoptosis; positive regulation of apoptosis; stress-activated MAPK cascade; rhythmic process; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of protein localization; regulation of transcription factor activity; JNK cascade; response to stress; negative regulation of protein binding; toll-like receptor 4 signaling pathway; response to UV; regulation of histone deacetylation; ossification; MyD88-independent toll-like receptor signaling pathway; peptidyl-threonine phosphorylation; regulation of circadian rhythm; toll-like receptor 2 signaling pathway; JUN phosphorylation; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cadmium ion; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; negative regulation of apoptosis
Research Articles on MAPK8
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Product Notes
The MAPK8 mapk8 (Catalog #AAA821367) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-JNK1/2/3 (pT183) Phospho Antibody reacts with Human, Mouse, Rat, Bovine, Chicken, Pig and may cross-react with other species as described in the data sheet. AAA Biotech's JNK1/2/3 (pT183) can be used in a range of immunoassay formats including, but not limited to, WB, IH, IF/IC. WB (1/500 - 1/1000)IH (1/100 - 1/200)
IF/IC (1/100 - 1/500). Researchers should empirically determine the suitability of the MAPK8 mapk8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JNK1/2/3 (pT183), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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