Western Blot (WB)
(Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml; 5 min) and from untreated HEL cells, using MBS6008461 (upper) or total Jak2 XP Rabbit mAb (lower). HEL cells contain a V617F activating mutation in Jak2.)
Rabbit JAK2 Monoclonal Antibody | anti-jak2b antibody
JAK2, phosphorylated (Tyr1007) Tyrosine-protein Kinase JAK2, Janus Kinase 2, JAK-2)
Gene Names
jak2b; jak2; JAK2bCA
Reactivity
Human, Monkey, Mouse, Rat
Applications
Western Blot
Purity
SupernatantSupernatant
Synonyms
JAK2; Monoclonal Antibody; phosphorylated (Tyr1007) Tyrosine-protein Kinase JAK2; Janus Kinase 2; JAK-2); Anti -JAK2; anti-jak2b antibody
Host
Rabbit
Reactivity
Human, Monkey, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG
Clone Number
D15E2
Specificity
Recognizes endogenous levels of human Jak2 when phosphorylated at Tyr1007. It can also detect double phosphorylation at Tyr1007 and Tyr1008. Cross-reactivity may be observed with phosphorylated Jak3 and Tyk2. Species Crossreactivity: Monkey, mouse and rat.
Purity/Purification
Supernatant
Supernatant
Supernatant
Form/Format
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Applicable Applications for anti-jak2b antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000. Incubate membrne with diluted antibody in 5% w/v BSA, 1x TBS, 0.1% Tween -20 at 4 degree C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Dilution: Western Blot: 1:1000. Incubate membrne with diluted antibody in 5% w/v BSA, 1x TBS, 0.1% Tween -20 at 4 degree C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Tyr1007 of human Jak2.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml; 5 min) and from untreated HEL cells, using MBS6008461 (upper) or total Jak2 XP Rabbit mAb (lower). HEL cells contain a V617F activating mutation in Jak2.)
Western Blot (WB)
(Western blot analysis of extracts from UT-7 cells, untreated or treated with erythropoietin (EPO; 3 units/ml; 5 min) and from untreated HEL cells, using MBS6008461 (upper) or total Jak2 XP Rabbit mAb (lower). HEL cells contain a V617F activating mutation in Jak2.)
Related Product Information for anti-jak2b antibody
Members of the Janus family of tyrosine kinases (Jak1, Jak2, Jak3 and Tyk2) are activated by ligands binding to a number of associated cytokine receptors (1). Upon cytokine receptor activation, Jak proteins become autophosphorylated and phosphorylate their associated receptors to provide multiple binding sites for signaling proteins. These associated signaling proteins, such as Stats (2), Shc (3), insulin receptor substrates (4) and focal adhesion kinase (FAK) (5), typically contain SH2 or other phospho-tyrosine-binding domains.
Product Categories/Family for anti-jak2b antibody
NCBI and Uniprot Product Information
Research Articles on jak2b
Similar Products
Product Notes
The jak2b (Catalog #AAA6008461) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JAK2, phosphorylated (Tyr1007) Tyrosine-protein Kinase JAK2, Janus Kinase 2, JAK-2) reacts with Human, Monkey, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JAK2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1:1000. Incubate membrne with diluted antibody in 5% w/v BSA, 1x TBS, 0.1% Tween -20 at 4 degree C with gentle shaking, overnight. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the jak2b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JAK2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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