Rabbit anti-Human, Mouse IDH3A Polyclonal Antibody | anti-IDH3A antibody

IDH3A Polyclonal Antibody

Cat. Num. AAA2555435

• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purification
• Synonyms: IDH3A; Polyclonal Antibody; IDH3A Polyclonal Antibody; H IDH alpha; HIDH alpha; Isocitrate dehydrogenase (NAD+) alpha chain precursor; Isocitrate dehydrogenase [NAD] subunit alpha; Isocitrate dehydrogenase [NAD] subunit alpha mitochondrial; Isocitrate dehydrogenase 3 (NAD+) alpha; Isocitrate dehydrogenase; Isocitric dehydrogenase; Isocitric dehydrogenase subunit alpha; mitochondrial; NAD; NAD(+)-specific ICDH subunit alpha; NAD(H) specific isocitrate dehydrogenase alpha subunit precursor; NAD+ specific ICDH; NADsubunit alpha mitochondrial precursor; OTTHUMP00000184906; anti-IDH3A antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Affinity Purification
• Form/Format: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 288

• Applicable Applications for anti-IDH3A antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: Recombinant protein of human IDH3A
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using IDH3A antibody.)

Related Product Information for anti-IDH3A antibody

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.

Product Categories/Family for anti-IDH3A antibody

Cancer; Metabolism; Signal transduction; Tags & Cell Markers

NCBI and Uniprot Product Information

• NCBI GI #: 5031777
• NCBI GeneID: 3419
• NCBI Accession #: NP_005521.1
• NCBI GenBank Nucleotide #: NM_005530.3
• UniProt Accession #: P50213; Q9H3X0; D3DW83
• Molecular Weight: Calculated: 31kDa; 40kDa; Observed: 40kDa
• NCBI Official Full Name: isocitrate dehydrogenase
• NCBI Official Synonym Full Names: isocitrate dehydrogenase 3 (NAD(+)) alpha
• NCBI Official Symbol: IDH3A
• NCBI Protein Information: isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
• UniProt Protein Name: Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial
• Protein Family: Isocitrate dehydrogenase
• UniProt Gene Name: IDH3A

NCBI Description

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle. Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit. The protein encoded by this gene is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase. [provided by RefSeq, Jul 2008]

Uniprot Description

Catalytic subunit of the enzyme which catalyzes the decarboxylation of isocitrate (ICT) into alpha-ketoglutarate. The heterodimer composed of the alpha (IDH3A) and beta (IDH3B) subunits and the heterodimer composed of the alpha (IDH3A) and gamma (IDH3G) subunits, have considerable basal activity but the full activity of the heterotetramer (containing two subunits of IDH3A, one of IDH3B and one of IDH3G) requires the assembly and cooperative function of both heterodimers.