Rabbit ZDHHC9 Polyclonal Antibody | anti-ZDHHC9 antibody

ZDHHC9 (K364) Polyclonal Antibody

Cat. Num. AAA3003677

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ZDHHC9; Polyclonal Antibody; ZDHHC9 (K364) Polyclonal Antibody; Palmitoyltransferase ZDHHC9; Zinc finger DHHC domain-containing protein 9; DHHC-9; DHHC9; Zinc finger protein 379; Zinc finger protein 380; CXorf11; ZDHHC10; ZNF379; ZNF380; CGI-89; UNQ261/PRO298; anti-ZDHHC9 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ZDHHC9 (K364) polyclonal antibody detects endogenous levels of ZDHHC9 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 364

• Applicable Applications for anti-ZDHHC9 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 332-380 of Human ZDHHC9.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of DHHC-9 (K364) polyclonal antibody at 1:500 dilutionLane1:Hela cell lysateLane2:Mouse kidney tissue lysateLane3:Rat brain tissue lysate)

Western Blot (WB)

(Western Blot (WB) analysis of ZDHHC9 (K364) pAb at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:A549 whole cell lysate (40ug) Lane3:H1792 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug))

Related Product Information for anti-ZDHHC9 antibody

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. ZDHHC9 (zinc finger, DHHC-type containing 9), also known as DHHC9, CGI-89, ZNF379 or CXorf11, is a 364 amino acid protein that localizes to the membrane of the endoplasmic reticulum and contains one DHHC-type zinc finger. Expressed at high levels in brain, lung, kidney, liver and skeletal muscle, ZDHHC9 exists in a complex with GOLGA7 and, via its DHHC domain, functions as a palmitoyltransferase that is specific for H-Ras and N-Ras. Mutations in the gene encoding ZDHHC9 are associated with X-linked mental retardation and colorectal cancer, the latter of which suggests a role in tumorigenesis.

NCBI and Uniprot Product Information

• NCBI GI #: 56682972
• NCBI Accession #: NP_057116.2
• NCBI GenBank Nucleotide #: NP_057116.2
• UniProt Accession #: Q9Y397
• Molecular Weight: ~ 41kDa
• NCBI Official Full Name: palmitoyltransferase ZDHHC9
• UniProt Protein Name: Palmitoyltransferase ZDHHC9
• Protein Family: Palmitoyltransferase
• UniProt Gene Name: ZDHHC9
• UniProt Synonym Gene Names: CXorf11; ZDHHC10; ZNF379; ZNF380; DHHC-9; DHHC9
• UniProt Entry Name: ZDHC9_HUMAN

Uniprot Description

ZDHHC9: The ZDHHC9-GOLGA7 complex is a palmitoyltransferase specific for HRAS and NRAS. Defects in ZDHHC9 are the cause of mental retardation syndromic X-linked ZDHHC9-related (MRXSZ). A disorder characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. Some patients have marfanoid habitus as an additional feature. Belongs to the DHHC palmitoyltransferase family. ERF2/ZDHHC9 subfamily.

Protein type: EC 2.3.1.225; Membrane protein, multi-pass; Membrane protein, integral; Transferase

Chromosomal Location of Human Ortholog: Xq26.1

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; endoplasmic reticulum; cytoplasm; intrinsic to Golgi membrane; integral to membrane

Molecular Function: zinc ion binding; Ras palmitoyltransferase activity; palmitoyltransferase activity; protein-cysteine S-palmitoleyltransferase activity

Biological Process: peptidyl-S-palmitoyl-L-cysteine biosynthetic process from peptidyl-cysteine; protein palmitoylation

Disease: Mental Retardation, X-linked, Syndromic, Raymond Type