Rabbit IRF7 Polyclonal Antibody | anti-IRF7 antibody

RABBIT ANTI IRF7 (C-TERMINAL)

Cat. Num. AAA223073

• Gene Names: IRF7; IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
• Applications: Western Blot
• Synonyms: IRF7; Polyclonal Antibody; RABBIT ANTI IRF7 (C-TERMINAL); anti-IRF7 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: This item detects an epitope within the C-terminal (CT) of human interferon regulatory factor 7 (IRF7), a 54kD transcriptional activator of type I interferons (IFNalpha/beta) in response to local and systemic viral infection. IRF7 is mostly expressed by plasmacytoid dendritic cells (pDC), which differentiate in response to IFNs and virus infection. This then stabilizes the IRF7 protein, in turn leading to increased IFN production. ; ; Multiple IRF7 transcript variants have been identified, although their individual functions have not yet been established.
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 503

• Applicable Applications for anti-IRF7 antibody: Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 51kDa in 293 whole cell lysate.; Western Blotting: Minimum Dilution: 1.0; Maximum Dilution: 2.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide
• Immunogen: 14 amino acid peptide sequence near the carboxy terminus of human IRF7.; Buffer Solution
• Antiserum Preparation: Antisera to human IRF7 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of whole cell lysates from HEK293 human kidney epithelial cells probed with Rabbit anti Human IRF7 (MBS221469) at 0.5(A), 1(B) and 2(C) ug/ml)

Testing Data #2

(Immunohistochemical staining of mouse spleen with Rabbit anti Human IRF7 (MBS221469))

Related Product Information for anti-IRF7 antibody

MBS221469 detects an epitope within the C-terminal (CT) of human interferon regulatory factor 7 (IRF7), a 54kD transcriptional activator of type I interferons (IFNalpha/beta) in response to local and systemic viral infection. IRF7 is mostly expressed by plasmacytoid dendritic cells (pDC), which differentiate in response to IFNs and virus infection. This then stabilizes the IRF7 protein, in turn leading to increased IFN production. Multiple IRF7 transcript variants have been identified, although their individual functions have not yet been established.

NCBI and Uniprot Product Information

• NCBI GI #: 4758610
• NCBI GeneID: 3665
• NCBI Accession #: NP_001563.2
• NCBI GenBank Nucleotide #: NM_001572.3
• UniProt Accession #: Q92985; O00331; O00332; O00333; O75924; Q9UE79; B9EGL3
• Molecular Weight: 55,635 Da
• NCBI Official Full Name: interferon regulatory factor 7 isoform a
• NCBI Official Synonym Full Names: interferon regulatory factor 7
• NCBI Official Symbol: IRF7
• NCBI Official Synonym Symbols: IRF7A; IRF7B; IRF7C; IRF7H; IRF-7H
• NCBI Protein Information: interferon regulatory factor 7; IRF-7; interferon regulatory factor-7H
• UniProt Protein Name: Interferon regulatory factor 7
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF7
• UniProt Synonym Gene Names: IRF-7
• UniProt Entry Name: IRF7_HUMAN

NCBI Description

IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established. [provided by RefSeq, Jul 2008]

Uniprot Description

IRF7: Key transcriptional regulator of type I interferon (IFN)-dependent immune responses and plays a critical role in the innate immune response against DNA and RNA viruses. Regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. Can efficiently activate both the IFN-beta (IFNB) and the IFN-alpha (IFNA) genes and mediate their induction via both the virus-activated, MyD88- independent pathway and the TLR-activated, MyD88-dependent pathway. Required during both the early and late phases of the IFN gene induction but is more critical for the late than for the early phase. Exists in an inactive form in the cytoplasm of uninfected cells and following viral infection, double-stranded RNA (dsRNA), or toll-like receptor (TLR) signaling, becomes phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change, leading to its dimerization and nuclear localization where along with other coactivators it can activate transcription of the type I IFN and ISG genes. Can also play a role in regulating adaptive immune responses by inducing PSMB9/LMP2 expression, either directly or through induction of IRF1. Binds to the Q promoter (Qp) of EBV nuclear antigen 1 a (EBNA1) and may play a role in the regulation of EBV latency. Can activate distinct gene expression programs in macrophages and regulate the anti-tumor properties of primary macrophages. Monomer. Homodimer; phosphorylation-induced. Heterodimer with IRF3. Interacts with TICAM1 and TICAM2. Interacts with rotavirus A NSP1; this interaction leads to the proteasome- dependent degradation of IRF7. Interacts with Epstein-Barr virus LF2. Interacts with MYD88 AND TRAF6. By type I interferon (IFN) and viruses. Expressed predominantly in spleen, thymus and peripheral blood leukocytes. In the absence of viral infection, maintained as a monomer in an autoinhibited state and phosphorylation disrupts this autoinhibition leading to the liberation of the DNA- binding and dimerization activities and its nuclear localization where it can activate type I IFN and ISG genes. Belongs to the IRF family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 11p15.5

Cellular Component: nucleoplasm; cytoplasm; endosome membrane; cytosol; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: transcription from RNA polymerase II promoter; regulation of interferon type I production; regulation of immune response; positive regulation of transcription, DNA-dependent; response to virus; cytokine and chemokine mediated signaling pathway; MyD88-independent toll-like receptor signaling pathway; establishment of viral latency; toll-like receptor 3 signaling pathway; negative regulation of transcription from RNA polymerase II promoter; interferon-alpha production; regulation of MyD88-dependent toll-like receptor signaling pathway; regulation of monocyte differentiation; regulation of MyD88-independent toll-like receptor signaling pathway; regulation of adaptive immune response; toll-like receptor signaling pathway; positive regulation of interferon-beta production; positive regulation of interferon type I production; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; immunoglobulin mediated immune response; interferon type I biosynthetic process; response to DNA damage stimulus; toll-like receptor 4 signaling pathway; positive regulation of interferon-alpha production; interferon-beta production

Disease: Immunodeficiency 39