Goat anti-Human IRF6 Polyclonal Antibody | anti-IRF6 antibody

IRF6, CT (Interferon regulatory factor 6, IRF-6)

Cat. Num. AAA649466

• Gene Names: IRF6; LPS; PIT; PPS; VWS; OFC6; PPS1; VWS1
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: IRF6; Polyclonal Antibody; CT (Interferon regulatory factor 6; IRF-6); Anti -IRF6; anti-IRF6 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: Recognizes human CPT1B. Species sequence homology: Mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.

• Applicable Applications for anti-IRF6 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Suitable for use in Western Blot, ELISA and Immunohistochemistry.; Dilution: Western Blot: 0.2-1ug/ml; Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
• Immunogen: Synthetic peptide corresponding to C-TPSMQLPPALPPQ from human IRF6 at C-terminal.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Immunohistochemistry (IHC)

(Human Kidney (formalin-fixed, paraffin-embedded) stained with IRF6 antibody at 2.5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Immunohistochemistry (IHC)

(Human Prostate (formalin-fixed, paraffin-embedded) stained with IRF6 antibody at 2.5ug/ml followed by biotinylated goat IgG secondary antibody alkaline phosphatase-streptavidin and chromogen.)

Western Blot (WB)

(Antibody staining (0.2ug/ml) of Human Ovary lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.)

Related Product Information for anti-IRF6 antibody

IRF6 is a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. Mutations can cause van der Woude syndrome and popliteal pterygium syndrome. This protein is involved in palate formation.

Product Categories/Family for anti-IRF6 antibody

Antibodies; Abs to Interferon

NCBI and Uniprot Product Information

• NCBI GI #: 331999978
• NCBI GeneID: 3664
• NCBI Accession #: NP_001193625.1
• NCBI GenBank Nucleotide #: NM_001206696.1
• UniProt Accession #: O14896; B4DLE2; D3DT90; F5GWX8; G0ZTL0
• Molecular Weight: 53,130 Da
• NCBI Official Full Name: interferon regulatory factor 6 isoform 2
• NCBI Official Synonym Full Names: interferon regulatory factor 6
• NCBI Official Symbol: IRF6
• NCBI Official Synonym Symbols: LPS; PIT; PPS; VWS; OFC6; PPS1; VWS1
• NCBI Protein Information: interferon regulatory factor 6
• UniProt Protein Name: Interferon regulatory factor 6
• Protein Family: Interferon regulatory factor
• UniProt Gene Name: IRF6
• UniProt Synonym Gene Names: IRF-6
• UniProt Entry Name: IRF6_HUMAN

NCBI Description

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2011]

Uniprot Description

Function: Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development

By similarity. Plays a role in regulating mammary epithelial cell proliferation

By similarity. May regulate WDR65 transcription

By similarity.

Subunit structure: Interacts with SERPINB5. Ref.7

Subcellular location: Nucleus

Potential. Cytoplasm. Note: Translocates to nucleus in response to an activating signal

By similarity. Ref.7 Ref.8

Tissue specificity: Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas. Ref.7

Post-translational modification: Phosphorylated. Phosphorylation status depends on the cell cycle and is a signal for ubiquitination and proteasome-mediated degradation. Ref.7 Ref.8

Involvement in disease: Van der Woude syndrome 1 (VWS1) [MIM:119300]: An autosomal dominant developmental disorder characterized by lower lip pits, cleft lip and/or cleft palate.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Ref.11 Ref.12 Ref.13 Ref.14 Ref.16 Ref.17 Ref.18Popliteal pterygium syndrome (PPS) [MIM:119500]: An autosomal dominant disorder characterized by oro-facial, skin and genital anomalies. Expressivity is variable. Clinical features include cleft lip/palate, lower lip cysts, syngnathia, congenital ankyloblepharon filiforme in some cases, bifid scrotum, hypoplastic scrotum, hypoplastic uterus, talipes equinovarus.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.10 Ref.19Non-syndromic orofacial cleft 6 (OFC6) [MIM:608864]: A birth defect consisting of cleft lips with or without cleft palate. Cleft lips are associated with cleft palate in two-third of cases. A cleft lip can occur on one or both sides and range in severity from a simple notch in the upper lip to a complete opening in the lip extending into the floor of the nostril and involving the upper gum.Note: Disease susceptibility is associated with variations affecting the gene represented in this entry. Ref.15 Ref.20

Sequence similarities: Belongs to the IRF family.Contains 1 IRF tryptophan pentad repeat DNA-binding domain.