Rabbit anti-Human IRE1p Polyclonal Antibody | anti-IRE1 antibody

IRE1p, NT (Inositol-Requiring Protein 1, IRE1a, Endoplasmic Reticulum-to-Nucleus Signaling 1, ERN1)

Cat. Num. AAA610790

• Gene Names: IRE1; ERN1
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: IRE1p; Polyclonal Antibody; NT (Inositol-Requiring Protein 1; IRE1a; Endoplasmic Reticulum-to-Nucleus Signaling 1; ERN1); Anti -IRE1p; anti-IRE1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IRE1p.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a liquid in PBS pH 7.2, 0.02% sodium azide.

• Applicable Applications for anti-IRE1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1-2ug/ml; Optimal dilutions to be determined by researcher.
• Immunogen: 15 amino acid peptide from near the amino terminus of human IRE1p (Genbank accession No. O75460).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of IRE1p in Raji cell lysate with IRE1p antibody at (A) 1ug/ml and (B) 2ug/ml.)

Related Product Information for anti-IRE1 antibody

Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94 (1,2). These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 (3,4) but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation (5). Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER (6). Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-IRE1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 6321870
• NCBI GeneID: 856478
• NCBI Accession #: NP_011946.1
• NCBI GenBank Nucleotide #: NM_001179209.1
• UniProt Accession #: P32361; D3DL29
• Molecular Weight: 126,976 Da
• NCBI Official Full Name: Ire1p
• NCBI Official Symbol: IRE1
• NCBI Official Synonym Symbols: ERN1
• NCBI Protein Information: Ire1p
• UniProt Protein Name: Serine/threonine-protein kinase/endoribonuclease IRE1
• Protein Family: Serine/threonine-protein kinase/endoribonuclease
• UniProt Gene Name: IRE1
• UniProt Synonym Gene Names: ERN1
• UniProt Entry Name: IRE1_YEAST

Uniprot Description

Function: Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain which leads to enzyme auto-activation. The active endoribonuclease domain splices HAC1 precursor mRNA to produce the mature form which then induces transcription of UPR target genes. Ref.5 Ref.6 Ref.7

Catalytic activity: ATP + a protein = ADP + a phosphoprotein.

Cofactor: Magnesium.

Enzyme regulation: The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ref.5

Subunit structure: Homodimer; in response to the accumulation of unfolded proteins. Ref.5 Ref.6

Subcellular location: Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Post-translational modification: Autophosphorylated mainly on serine residues. Ref.6

Miscellaneous: Present with 259 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.Contains 1 KEN domain.Contains 1 protein kinase domain.

Sequence caution: The sequence AAB68894.1 differs from that shown. Reason: Erroneous initiation.