Rabbit IRE1p Polyclonal Antibody | anti-IRE1p antibody

IRE1p Antibody

Cat. Num. AAA9406238

• Gene Names: ERN1; IRE1; IRE1P; IRE1a; hIRE1p
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: IRE1p; Polyclonal Antibody; IRE1p Antibody; Endoplasmic reticulum-to-nucleus signaling 1; ERN1; anti-IRE1p antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 977

• Applicable Applications for anti-IRE1p antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 15 amino acid peptide from near the amino terminus of human IRE1p.
• Target Name: IRE1p
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of IRE1p in A-20 cell lysate with IRE1p antibody at (A) 0.5, (B) 1 and (C) 2 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of IRE1p in A20 cells with IRE1p antibody at 1 ug/mL.)

Related Product Information for anti-IRE1p antibody

Accumulation of malfolded proteins in the endoplasmic reticulum (ER) activates the unfolded protein response (UPR) and the upregulation of the ER molecular chaperones GRP78 and GRP 94. These proteins are normally bound to ER transmembrane proteins such as IRE1p and ATF6 but ER stress causes their dissociation. This allows IRE1p, a serine-threonine protein kinase to transduce the unfolded protein signal from the ER to the nucleus. IRE1p also has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-IRE1p antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 153946421
• NCBI GeneID: 2081
• NCBI Accession #: NP_001424.3
• NCBI GenBank Nucleotide #: NM_001433.3
• UniProt Accession #: O75460; Q59EE2; A1L457; A8K8N8; A8MXS7
• Molecular Weight: 6,649 Da
• NCBI Official Full Name: serine/threonine-protein kinase/endoribonuclease IRE1
• NCBI Official Synonym Full Names: endoplasmic reticulum to nucleus signaling 1
• NCBI Official Symbol: ERN1
• NCBI Official Synonym Symbols: IRE1; IRE1P; IRE1a; hIRE1p
• NCBI Protein Information: serine/threonine-protein kinase/endoribonuclease IRE1
• UniProt Protein Name: Serine/threonine-protein kinase/endoribonuclease IRE1
• UniProt Gene Name: ERN1
• UniProt Synonym Gene Names: hIRE1p; IRE1a
• UniProt Entry Name: ERN1_HUMAN

NCBI Description

The protein encoded by this gene is the ER to nucleus signalling 1 protein, a human homologue of the yeast Ire1 gene product. This protein possesses intrinsic kinase activity and an endoribonuclease activity and it is important in altering gene expression as a response to endoplasmic reticulum-based stress signals. [provided by RefSeq, Jul 2008]

Uniprot Description

IRE1: a ser/thr protein kinase that possess endonuclease activity. Important in altering gene expression as a response to endoplasmic reticulum based stress signals. Senses unfolded proteins in the lumen of the endoplasmic reticulum via its N-terminal domain, which leads to enzyme auto-activation. The active endoribonuclease domain splices XBP1 mRNA to generate a new C-terminus, converting it into a potent unfolded-protein response transcriptional activator and triggering growth arrest and apoptosis. The kinase domain is activated by trans-autophosphorylation. Kinase activity is required for activation of the endoribonuclease domain. Ubiquitously expressed. High levels observed in pancreatic tissue. Functionally connected with insulin biosynthesis in pancreatic beta cells. Homodimer; disulfide-linked. Dimer formation is driven by hydrophobic interactions within the N-terminal luminal domains and stabilized by disulfide bridges. Also binds HSPA5, a negative regulator of the unfolded protein response. This interaction may disrupt homodimerization and prevent activation of IRE1.

Protein type: Endoplasmic reticulum; Membrane protein, integral; Chaperone; Protein kinase, Other; Ribonuclease; Protein kinase, Ser/Thr (non-receptor); Apoptosis; EC 2.7.11.1; Kinase, protein; Other group; IRE family

Chromosomal Location of Human Ortholog: 17q24.2

Cellular Component: endoplasmic reticulum membrane; mitochondrion; endoplasmic reticulum; cytoplasm; integral to endoplasmic reticulum membrane; nuclear inner membrane

Molecular Function: endoribonuclease activity; identical protein binding; protein serine/threonine kinase activity; protein binding; protein homodimerization activity; enzyme binding; magnesium ion binding; ADP binding; ATP binding

Biological Process: transcription, DNA-dependent; unfolded protein response; protein amino acid autophosphorylation; RNA splicing; protein amino acid phosphorylation; positive regulation of RNA splicing; cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; regulation of transcription, DNA-dependent; endothelial cell proliferation; UFP-specific transcription factor mRNA processing during unfolded protein response; mRNA catabolic process; cell cycle arrest; regulation of macroautophagy; mRNA cleavage