Heparanase (HPA) Recombinant Protein | HPA recombinant protein

Recombinant Heparanase (HPA)

Cat. Num. AAA2010395

• Gene Names: HPSE; HPA; HPA1; HPR1; HSE1; HPSE1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Heparanase (HPA); Recombinant Heparanase (HPA); HPA recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF- PSFLSVT IDANLATDPR FLILLGSPKL RTLARGLSPA YLRFGGTKTD FLIFDPKKES TFEERSYWQS QVNQDICKYG SIPPDVEEKL RLEWPYQEQL LLREHYQKKF KNSTYSRSSV DVLYTFANCS GLDLIFGLNA LLRTADLQWN SSNAQLLLDY CSSKGYNISW ELGNEPNSFL KKADIFINGS QLGEDFIQLH KLLRKSTFKN AKLYGPDVGQ PRRKTAKM
• Sequence Length: 543

• Applicable Applications for HPA recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Pro54~Met278 (Accession # Q9Y251) with N-terminal His-Tag
• Subcellular Location: Lysosome membrane; Peripheral membrane protein. Secreted. Nucleus
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 9
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 148746204
• NCBI GeneID: 10855
• NCBI Accession #: NP_001092010.1
• NCBI GenBank Nucleotide #: NM_001098540.2
• UniProt Accession #: Q9Y251; Q53GE5; Q9UL39; A9JIG7; C7F7I3; E9PCA9; E9PGR1
• Molecular Weight: 27.3kDa
• NCBI Official Full Name: heparanase isoform 1 preproprotein
• NCBI Official Synonym Full Names: heparanase
• NCBI Official Symbol: HPSE
• NCBI Official Synonym Symbols: HPA; HPA1; HPR1; HSE1; HPSE1
• NCBI Protein Information: heparanase; heparanase-1; endo-glucoronidase
• UniProt Protein Name: Heparanase
• UniProt Gene Name: HPSE
• UniProt Synonym Gene Names: HEP; HPA; HPA1; HPR1; HPSE1; HSE1; Hpa1
• UniProt Entry Name: HPSE_HUMAN

NCBI Description

Heparan sulfate proteoglycans are major components of the basement membrane and extracellular matrix. The protein encoded by this gene is an enzyme that cleaves heparan sulfate proteoglycans to permit cell movement through remodeling of the extracellular matrix. In addition, this cleavage can release bioactive molecules from the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

HPSE: Endoglycosidase that cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Participates in extracellular matrix (ECM) degradation and remodeling. Selectively cleaves the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying either a 3-O-sulfo or a 6-O-sulfo group. Can also cleave the linkage between a glucuronic acid unit and an N-sulfo glucosamine unit carrying a 2-O-sulfo group, but not linkages between a glucuronic acid unit and a 2-O-sulfated iduronic acid moiety. It is essentially inactive at neutral pH but becomes active under acidic conditions such as during tumor invasion and in inflammatory processes. Facilitates cell migration associated with metastasis, wound healing and inflammation. Enhances shedding of syndecans, and increases endothelial invasion and angiogenesis in myelomas. Acts as procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Increases cell adhesion to the extacellular matrix (ECM), independent of its enzymatic activity. Induces AKT1/PKB phosphorylation via lipid rafts increasing cell mobility and invasion. Heparin increases this AKT1/PKB activation. Regulates osteogenesis. Enhances angiogenesis through up- regulation of SRC-mediated activation of VEGF. Implicated in hair follicle inner root sheath differentiation and hair homeostasis. Belongs to the glycosyl hydrolase 79 family.

Protein type: Secreted, signal peptide; Hydrolase; Secreted; Glycan Metabolism - glycosaminoglycan degradation; EC 3.2.1.166; Extracellular matrix

Chromosomal Location of Human Ortholog: 4q21.3

Cellular Component: nucleoplasm; lysosomal lumen; intracellular membrane-bound organelle; lysosomal membrane; lysosome; extracellular region; nucleus; lipid raft

Molecular Function: syndecan binding; protein dimerization activity; protein binding; beta-glucuronidase activity; heparanase activity

Biological Process: positive regulation of hair follicle development; proteoglycan metabolic process; positive regulation of protein kinase B signaling cascade; glycosaminoglycan catabolic process; heparan sulfate proteoglycan catabolic process; positive regulation of blood coagulation; glycosaminoglycan metabolic process; cell-matrix adhesion; positive regulation of osteoblast proliferation; carbohydrate metabolic process; pathogenesis; regulation of hair follicle development