Western Blot (WB)
(Sample Data: Western blot analysis of IRAK4 in HeLa cell lysate with IRAK4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) ug/ml, respectively.)
Rabbit anti-Human IRAK4 Polyclonal Antibody | anti-IRAK4 antibody
Anti-IRAK4
Reactivity
Human
Applications
Western Blot, ELISA, Immunocytochemistry, Immunofluorescence
Purity
Affinity chromatography
Synonyms
IRAK4; Polyclonal Antibody; Anti-IRAK4; anti-IRAK4 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the IRAK4 protein and has no cross response to other IRAKs
Purity/Purification
Affinity chromatography
Form/Format
PBS + 0.02% sodium azide
Applicable Applications for anti-IRAK4 antibody
Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF)
Immunogen
IRAK4 antibody was raised against a synthetic peptide corresponding to amino acids at the carboxy terminus of human IRAK4
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
1 year at -20 degree C from date of shipment
1 year at -20 degree C from date of shipment
Western Blot (WB)
(Sample Data: Western blot analysis of IRAK4 in HeLa cell lysate with IRAK4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) ug/ml, respectively.)
Western Blot (WB)
(Sample Data: Western blot analysis of IRAK4 in HeLa cell lysate with IRAK4 antibody at 1 (lane 1), 2 (lane 2), and 4 (lane 3) ug/ml, respectively.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of IRAK4 in K562 cells with IRAK4 antibody at 10 ug/ml.)
Immunocytochemistry (ICC)
(Sample Data: Immunocytochemistry of IRAK4 in K562 cells with IRAK4 antibody at 10 ug/ml.)
Related Product Information for anti-IRAK4 antibody
Interleukin-1 receptor-associated kinase 4 (IRAK4) is an important mediator in the signal transduction of Toll-like receptor (TLR) and IL1R family members (1). IRAK4 is involved in the Toll-like receptor signaling pathway leading to Apoptosis. IRAK4 has molecular functions like protein binding, ATP binding, kinase activity and magnesium ion binding. Toll/IL-1 receptor family members like IRAK4 are central components of host defense mechanisms in a variety of species (2). One well conserved element in their signal transduction is the Ser/Thr kinase activity which couple early signaling events in a receptor complex at the plasma membrane to larger signalosomes in the cytosol.
Product Categories/Family for anti-IRAK4 antibody
References
1. Li, S. et al: IRAK-4: a novel member of the IRAK family with the properties of an IRAK-kinase. Proc. Natl. Acad. Sci. USA. 2002; 99:5567-72.
2. Suzuki, N. et al: IRAK-4 as the central TIR signaling mediator in innate immunity. Trends Immunol. 2002; 23:503-6.
2. Suzuki, N. et al: IRAK-4 as the central TIR signaling mediator in innate immunity. Trends Immunol. 2002; 23:503-6.
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Product Notes
The IRAK4 (Catalog #AAA516021) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IRAK4 reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IRAK4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunocytochemistry (ICC), Immunofluorescence (IF). Researchers should empirically determine the suitability of the IRAK4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IRAK4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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