Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP1R12B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Rabbit anti-Human PPP1R12B Polyclonal Antibody | anti-PPP1R12B antibody
PPP1R12B Rabbit pAb
Gene Names
PPP1R12B; MYPT2; PP1bp55
Reactivity
Human
Applications
Western Blot
Purity
Affinity purification
Synonyms
PPP1R12B; Polyclonal Antibody; PPP1R12B Rabbit pAb; MYPT2; PP1bp55; anti-PPP1R12B antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Sequence
TFSRSRAERQAQEQPREKPTDTEGLEGSPEKHEPSAVPATEAGEGQQPWGRSLDEEPICHRLRCPAQPDKPTTPASPSTSRPSLYTSSHLLWTNRFSVPDSESSETTTNTTTAKEMDKNENEEADLDEQSSKRLSIRERRRPKERRRGTGINFWTKDEDETDGSEEVKETWHERLSRLESGGSNPTTSDSYGDRASARARREAREARLATLTSRVEEDSNRDYKKLYESALTENQKLKTKLQEAQLELADIKSKLEKVAQQKQEKTSDRSS
Applicable Applications for anti-PPP1R12B antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 660-930 of human PPP1R12B (NP_002472.2).
Positive Samples
293T, U-87MG
Preparation and Storage
Store at -20 degree C. Avoid freeze/thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP1R12B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using PPP1R12B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
Related Product Information for anti-PPP1R12B antibody
Background: Myosin phosphatase is a protein complex comprised of three subunits: a catalytic subunit (PP1c-delta, protein phosphatase 1, catalytic subunit delta), a large regulatory subunit (MYPT, myosin phosphatase target) and small regulatory subunit (sm-M20). Two isoforms of MYPT have been isolated--MYPT1 and MYPT2, the first of which is widely expressed, and the second of which may be specific to heart, skeletal muscle, and brain. Each of the MYPT isoforms functions to bind PP1c-delta and increase phosphatase activity. This locus encodes both MYTP2 and M20. Alternatively spliced transcript variants encoding different isoforms have been identified. Related pseudogenes have been defined on the Y chromosome. [provided by RefSeq, Oct 2011]
Product Categories/Family for anti-PPP1R12B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
110,404 Da
NCBI Official Full Name
protein phosphatase 1 regulatory subunit 12B isoform a
NCBI Official Synonym Full Names
protein phosphatase 1, regulatory subunit 12B
NCBI Official Symbol
PPP1R12B
NCBI Official Synonym Symbols
MYPT2; PP1bp55
NCBI Protein Information
protein phosphatase 1 regulatory subunit 12B; myosin phosphatase target subunit 2; myosin phosphatase, target subunit 2; myosin phosphatase-targeting subunit 2; protein phosphatase 1, regulatory (inhibitor) subunit 12B
UniProt Protein Name
Protein phosphatase 1 regulatory subunit 12B
Protein Family
UniProt Gene Name
PPP1R12B
UniProt Synonym Gene Names
MYPT2; Myosin phosphatase target subunit 2
UniProt Entry Name
MYPT2_HUMAN
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Product Notes
The PPP1R12B ppp1r12b (Catalog #AAA9142806) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PPP1R12B Rabbit pAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PPP1R12B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the PPP1R12B ppp1r12b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: TFSRSRAERQ AQEQPREKPT DTEGLEGSPE KHEPSAVPAT EAGEGQQPWG RSLDEEPICH RLRCPAQPDK PTTPASPSTS RPSLYTSSHL LWTNRFSVPD SESSETTTNT TTAKEMDKNE NEEADLDEQS SKRLSIRERR RPKERRRGTG INFWTKDEDE TDGSEEVKET WHERLSRLES GGSNPTTSDS YGDRASARAR REAREARLAT LTSRVEEDSN RDYKKLYESA LTENQKLKTK LQEAQLELAD IKSKLEKVAQ QKQEKTSDRS S. It is sometimes possible for the material contained within the vial of "PPP1R12B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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