Human Secreted frizzled-related protein 2 ELISA Kit | SFRP2 elisa kit

Human Secreted frizzled-related protein 2 ELISA Kit

Cat. Num. AAA760706

• Gene Names: SFRP2; FRP-2; SARP1; SDF-5
• Reactivity: Human
• Synonyms: Secreted frizzled-related protein 2; Human Secreted frizzled-related protein 2 ELISA Kit; SFRP2 (Secreted frizzled-related protein 2)/SARP-1/SDF-5/FRP2/FRP-2/secreted apoptosis related protein 1/SARP1/sFRP-2/Secreted apoptosis-related protein 1; SFRP2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SFRP2. No significant cross-reactivity or interference between SFRP2 and analogues was observed.
• Sequence Length: 295

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.25-80ng/ml
• Sensitivity: 0.75ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFRP2 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 48475052
• NCBI GeneID: 6423
• NCBI Accession #: NP_003004.1
• NCBI GenBank Nucleotide #: NM_003013.2
• Molecular Weight: 33,490 Da
• NCBI Official Full Name: secreted frizzled-related protein 2
• NCBI Official Synonym Full Names: secreted frizzled-related protein 2
• NCBI Official Symbol: SFRP2
• NCBI Official Synonym Symbols: FRP-2; SARP1; SDF-5
• NCBI Protein Information: secreted frizzled-related protein 2
• UniProt Protein Name: Secreted frizzled-related protein 2
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP2
• UniProt Synonym Gene Names: FRP2; SARP1; FRP-2; sFRP-2; SARP-1
• UniProt Entry Name: SFRP2_HUMAN

NCBI Description

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. Methylation of this gene is a potential marker for the presence of colorectal cancer. [provided by RefSeq, Jul 2008]

Uniprot Description

SFRP2: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 4q31.3

Cellular Component: extracellular matrix; extracellular space; integral to membrane

Molecular Function: fibronectin binding; G-protein coupled receptor activity; integrin binding; receptor agonist activity; Wnt receptor activity; Wnt-protein binding

Biological Process: cell-cell signaling; cellular response to extracellular stimulus; chondrocyte development; collagen fibril organization; convergent extension involved in axis elongation; digestive tract morphogenesis; embryonic digit morphogenesis; G-protein coupled receptor protein signaling pathway; male gonad development; negative regulation of BMP signaling pathway; negative regulation of caspase activity; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of JNK activity; negative regulation of mesodermal cell fate specification; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of Wnt receptor signaling pathway; patterning of blood vessels; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of cell adhesion mediated by integrin; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of osteoblast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; response to drug; response to nutrient; Wnt receptor signaling pathway through beta-catenin