Rabbit anti-Human IRAK1 Polyclonal Antibody | anti-IRAK1 antibody

IRAK1 Antibody

Cat. Num. AAA9402013

• Gene Names: IRAK1; IRAK; pelle
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Synonyms: IRAK1; Polyclonal Antibody; IRAK1 Antibody; EC 2.7.11.1; IL1RAK; Interleukin-1 receptor-associated kinase 1; IRAK; IRAK-1; anti-IRAK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous levels of total IRAK1 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 682

• Applicable Applications for anti-IRAK1 antibody: Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry: 1:50~1:100
• Immunogen Type: Peptide
• Immunogen Description: Synthesized peptide derived from Internal of human IRAK1.
• Target Name: IRAK1
• Preparation and Storage: Store at -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of paraffin-embedded human brain tissue using IRAK1 antibody.)

Related Product Information for anti-IRAK1 antibody

Serine/threonine-protein kinase that plays a critical role in initiating innate immune response against foreign pathogens. Involved in Toll-like receptor (TLR) and IL-1R signaling pathways. Is rapidly recruited by MYD88 to the receptor-signaling complex upon TLR activation. Association with MYD88 leads to IRAK1 phosphorylation by IRAK4 and subsequent autophosphorylation and kinase activation. Phosphorylates E3 ubiquitin ligases Pellino proteins (PELI1, PELI2 and PELI3) to promote pellino-mediated polyubiquitination of IRAK1. Then, the ubiquitin-binding domain of IKBKG/NEMO binds to polyubiquitinated IRAK1 bringing together the IRAK1-MAP3K7/TAK1-TRAF6 complex and the NEMO-IKKA-IKKB complex. In turn, MAP3K7/TAK1 activates IKKs (CHUK/IKKA and IKBKB/IKKB) leading to NF-kappa-B nuclear translocation and activation. Alternatively, phosphorylates TIRAP to promote its ubiquitination and subsequent degradation. Phosphorylates the interferon regulatory factor 7 (IRF7) to induce its activation and translocation to the nucleus, resulting in transcriptional activation of type I IFN genes, which drive the cell in an antiviral state. When sumoylated, translocates to the nucleus and phosphorylates STAT3.Cao Z., Science 271:1128-1131(1996).Reichwald K., Mamm. Genome 11:182-190(2000).Jensen L.E., J. Biol. Chem. 276:29037-29044(2001).

Product Categories/Family for anti-IRAK1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 68800343
• NCBI GeneID: 3654
• NCBI Accession #: NP_001020413.1
• NCBI GenBank Nucleotide #: NM_001025242.1
• UniProt Accession #: P51617; Q7Z5V4; Q96C06; Q96RL2; D3DWW3; D3DWW4
• Molecular Weight: 68,022 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 1 isoform 2
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 1
• NCBI Official Symbol: IRAK1
• NCBI Official Synonym Symbols: IRAK; pelle
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK1
• UniProt Synonym Gene Names: IRAK; IRAK-1
• UniProt Entry Name: IRAK1_HUMAN

NCBI Description

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: Protein kinase, Ser/Thr (non-receptor); EC 2.7.11.1; Kinase, protein; Protein kinase, TKL; TKL group; IRAK family

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: interleukin-1 receptor complex; cytoplasm; plasma membrane; endosome membrane; lipid particle; nucleus; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; ubiquitin-protein ligase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; protein amino acid autophosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; stress-activated MAPK cascade; protein ubiquitination; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; positive regulation of MAP kinase activity; response to peptidoglycan; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; JNK cascade; toll-like receptor 4 signaling pathway; aging; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; regulation of cytokine and chemokine mediated signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; negative regulation of apoptosis