Rabbit anti-Human Irak1 Polyclonal Antibody | anti-IRAK1 antibody

Irak1, ID (Irak1, Il1rak, Interleukin-1 receptor-associated kinase 1, Pelle-like protein kinase)

Cat. Num. AAA6000633

• Gene Names: IRAK1; IRAK; pelle
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity Purified; Purified by immunoaffinity chromatography
• Synonyms: Irak1; Polyclonal Antibody; ID (Irak1; Il1rak; Interleukin-1 receptor-associated kinase 1; Pelle-like protein kinase); Anti -Irak1; anti-IRAK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IRAK1/IRAK.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography
• Form/Format: Supplied as a liquid in PBS, (without Mg2+, Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IRAK1 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: Immunohistochemistry (Paraffin): 1:100 requires HIER using sodium citrate buffer pH6.0; Optimal dilutions to be determined by the researcher.
• Immunogen: Synthetic peptide corresponding to human IRAK1/IRAK.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap,

Immunohistochemistry (IHC)

(Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE))

Western Blot (WB)

(Western blot analysis of extracts from NIH-3T3 cells, using IRAK1. The lane on the right is treated with the synthesized peptide.)

Related Product Information for anti-IRAK1 antibody

IRAK1, an IRAK type protein kinase, is a signal transducer for the immune response Toll-like receptor and interleukin-1 (IL-1) receptor signaling cascades. IRAK1 is recruited to the IL-1 receptor, phosphorylated and progressively degraded in response to IL-1 induction. The protein also has the ability to autophosphorylate. IRAK1 is immediately downstream of the MyD88 adapter protein and immediately upstream of TRAF6. IRAK1 (-/-) mice have impaired responses to interleukin-18. At least two mRNA transcript variants have been identified - 2.0 and 3.6 kb.

Product Categories/Family for anti-IRAK1 antibody

Antibodies; Abs to Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 68800350
• NCBI GeneID: 3654
• NCBI Accession #: NP_001020414.1
• NCBI GenBank Nucleotide #: NM_001025243.1
• UniProt Accession #: P51617; Q7Z5V4; Q96C06; Q96RL2; D3DWW3; D3DWW4
• Molecular Weight: 76,537 Da
• NCBI Official Full Name: interleukin-1 receptor-associated kinase 1 isoform 3
• NCBI Official Synonym Full Names: interleukin-1 receptor-associated kinase 1
• NCBI Official Symbol: IRAK1
• NCBI Official Synonym Symbols: IRAK; pelle
• NCBI Protein Information: interleukin-1 receptor-associated kinase 1; IRAK-1; Pelle homolog
• UniProt Protein Name: Interleukin-1 receptor-associated kinase 1
• Protein Family: Interleukin-1 receptor-associated kinase
• UniProt Gene Name: IRAK1
• UniProt Synonym Gene Names: IRAK; IRAK-1
• UniProt Entry Name: IRAK1_HUMAN

NCBI Description

This gene encodes the interleukin-1 receptor-associated kinase 1, one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This gene is partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

IRAK1: a TKL kinase of the IRAK family. Involved in Toll/IL-1 signaling. One of two putative serine/threonine kinases that become associated with the interleukin-1 receptor following IL-1 engagement, triggering intracellular signaling cascades leading to transcriptional up-regulation and mRNA stabilization. Extensively phosphorylated after its association with IL1-R-1. Polyubiquitinated; after cell stimulation with IL-1-beta. Polyubiquitination occurs with polyubiquitin chains linked through 'Lys-63'. Partially responsible for IL1-induced upregulation of the transcription factor NF-kappa B. Three isoforms of the human protein and produced by alternative splicing. Isoform 1 binds rapidly but is then degraded allowing isoform 2 to mediate a slower, more sustained response to the cytokine. Isoform 2 is inactive suggesting that the kinase activity of this enzyme is not required for IL-1 signaling. Once phosphorylated, IRAK1 recruits the adapter protein PELI1. Isoform 1 and isoform 2 are ubiquitously expressed in all tissues examined, with isoform 1 being more strongly expressed than isoform 2.

Protein type: Kinase, protein; Protein kinase, TKL; EC 2.7.11.1; Protein kinase, Ser/Thr (non-receptor); TKL group; IRAK family

Chromosomal Location of Human Ortholog: Xq28

Cellular Component: interleukin-1 receptor complex; cytoplasm; plasma membrane; endosome membrane; lipid particle; nucleus; cytosol

Molecular Function: NF-kappaB-inducing kinase activity; protein serine/threonine kinase activity; protein binding; interleukin-1 receptor binding; protein homodimerization activity; protein heterodimerization activity; heat shock protein binding; ubiquitin-protein ligase activity; kinase activity; ATP binding; protein kinase activity

Biological Process: nerve growth factor receptor signaling pathway; activation of MAPK activity; positive regulation of transcription, DNA-dependent; positive regulation of smooth muscle cell proliferation; protein amino acid autophosphorylation; stress-activated MAPK cascade; protein ubiquitination; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; signal transduction; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; activation of NF-kappaB transcription factor; toll-like receptor 5 signaling pathway; positive regulation of MAP kinase activity; response to peptidoglycan; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; JNK cascade; toll-like receptor 4 signaling pathway; aging; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; activation of NF-kappaB-inducing kinase; protein oligomerization; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; inhibition of NF-kappaB transcription factor; toll-like receptor signaling pathway; regulation of cytokine and chemokine mediated signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; negative regulation of apoptosis