Rabbit IP3R1 Polyclonal Antibody | anti-ITPR1 antibody

IP3R1 (ITPR1, inositol 1,4,5-triphosphate receptor, type 1, Inositol 1,4,5-trisphosphate receptor type 1, Insp3r1, InsP3R1, INSP3R1, IP3R, IP3R1, IP3 receptor isoform 1, SCA15, SCA16, Type 1 inositol 1,4,5-trisphosphate receptor, Type 1 InsP3 receptor)

Cat. Num. AAA611375

• Gene Names: ITPR1; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1
• Reactivity: Canine, Human, Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by epitope affinity chromatography.
• Synonyms: IP3R1; Polyclonal Antibody; IP3R1 (ITPR1; inositol 1; 4; 5-triphosphate receptor; type 1; Inositol 1; 5-trisphosphate receptor type 1; Insp3r1; InsP3R1; INSP3R1; IP3R; IP3 receptor isoform 1; SCA15; SCA16; Type 1 inositol 1; 5-trisphosphate receptor; Type 1 InsP3 receptor); Anti -IP3R1 (ITPR1; anti-ITPR1 antibody

• Host: Rabbit
• Reactivity: Canine, Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human IP3R-I (weak) at ~240kD. Species Crossreactivity: mouse, rat, canine.
• Purity/Purification: Affinity Purified; Purified by epitope affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.

• Applicable Applications for anti-ITPR1 antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunocytochemistry and Immunohistochemistry.; Dilution: Western Blot: 1:1000; Immunohistochemistry (Frozen): 1:250
• Immunogen: Synthetic peptide corresponding to aa1829-1848 NKKKDDEVDRDAPSRKKAKE of the cytoplasmic C-terminal domain of human IP3RI. The sequence is identical in mouse and rat.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Immunolocalization of IP3R-I in rat cerebellum using MBS611375.)

Related Product Information for anti-ITPR1 antibody

IP3R1 is a second messenger for many growth factors, hormones, and neuro-transmitters. Upon binding to the IP3 receptor (IP3R), IP3 triggers the release of intracellular, luminal calcium to the cytosol. Functional IP3R is a homo- or heterotetramer of ~240kD glycoprotein subunits, that is structurally reminiscent of members of the voltage-gated ion channel superfamily. IP3R protein is structurally and functionally related to one other important intracellular calcium-release channel, the ryanodine receptor. The similarity between the two receptors continues at the physiological level owing to a physical association each receptor can have with the immunophilin protein, FKBP12. Cytosolic calcium levels appear to be regulated by a feedback loop that starts with calcium activation of protein kinase C (PKC) and calcineurin, a protein phosphatase. Phosphorylation of the IP3R by PKC causes an increase in IP3-mediated calcium release. Concomitantly, the phosphatase activity of calcineurin is stimulated upon its association with the FKBP12-IP3R complex. Calcium release is reduced when the PKC target site on the IP3R is dephosphorylated by calcineurin resulting in calcium oscillations. Mammalian IP3R subunits are the product of three distinct genes that are widely expressed and differentially regulated. Type-I IP3R has been detected in heart, liver, kidney, ovary, and Purkinje neurons of the cerebellum. IP3R-II protein is found predominantly in the brain. Isoform III expression has been shown to occur in pancreatic islets, kidney and the gastrointestinal tract.

Product Categories/Family for anti-ITPR1 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 269954694
• NCBI GeneID: 3708
• NCBI Accession #: NP_001161744.1
• NCBI GenBank Nucleotide #: NM_001168272.1
• UniProt Accession #: Q14643; Q14660; Q99897; E7EPX7; E9PDE9
• Molecular Weight: 313,929 Da
• NCBI Official Full Name: inositol 1,4,5-trisphosphate receptor type 1 isoform 3
• NCBI Official Synonym Full Names: inositol 1,4,5-trisphosphate receptor, type 1
• NCBI Official Symbol: ITPR1
• NCBI Official Synonym Symbols: IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1
• NCBI Protein Information: inositol 1,4,5-trisphosphate receptor type 1; IP3R 1; IP3 receptor; type 1 InsP3 receptor; inositol 1,4,5-triphosphate receptor, type 1; type 1 inositol 1,4,5-trisphosphate receptor
• UniProt Protein Name: Inositol 1,4,5-trisphosphate receptor type 1
• Protein Family: Inositol 1,4,5-trisphosphate receptor
• UniProt Gene Name: ITPR1
• UniProt Synonym Gene Names: INSP3R1; IP3R 1; InsP3R1
• UniProt Entry Name: ITPR1_HUMAN

NCBI Description

This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]

Uniprot Description

IP3R1: a multi-pass endoplasmic reticulum membrane receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaMKII, eventually leading to the activation of downstream apoptosis pathways. Part of a cGMP kinase signaling complex that includes alpha-actin, calponin H1, phospholamban, PKG1 and IP3R1. Widely expressed. The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. Phosphorylation by PKA prevents the ligand-induced opening of the calcium channels. Belongs to the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family. Eight alternatively spliced isoforms have been described.

Protein type: Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p26.1

Cellular Component: endoplasmic reticulum membrane; membrane; sarcoplasmic reticulum; endoplasmic reticulum; postsynaptic density; nucleolus; integral to membrane; nuclear inner membrane; platelet dense granule membrane; calcineurin complex

Molecular Function: protein binding; calcium ion transmembrane transporter activity; calcium channel inhibitor activity; intracellular ligand-gated calcium channel activity; phosphoinositide binding; inositol 1,4,5-triphosphate-sensitive calcium-release channel activity

Biological Process: negative regulation of calcium-mediated signaling; epidermal growth factor receptor signaling pathway; platelet activation; voluntary musculoskeletal movement; inositol phosphate-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; signal transduction; post-embryonic development; endoplasmic reticulum calcium ion homeostasis; phospholipase C activation; release of sequestered calcium ion into cytosol; calcium ion transport; response to hypoxia; energy reserve metabolic process; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; regulation of insulin secretion