Western Blot (WB)
(Western Blot: Sample: Recombinant IL3Ra, Mouse.)
Rabbit anti-Mouse Interleukin 3 Receptor Alpha (IL3Ra) Polyclonal Antibody | anti-IL3Ra antibody
Polyclonal Antibody to Interleukin 3 Receptor Alpha (IL3Ra)
Gene Names
Il3ra; CD123; SUT-1; CDw123
Reactivity
Mouse
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Interleukin 3 Receptor Alpha (IL3Ra); Polyclonal Antibody; Polyclonal Antibody to Interleukin 3 Receptor Alpha (IL3Ra); anti-IL3Ra antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
Concentration
0.47mg/ml (varies by lot)
Applicable Applications for anti-IL3Ra antibody
WB; IHC; ICC; IP.
Application Notes
Western blotting: 0.5-2 ug/mL;1:230-940
Immunohistochemistry: 5-20 ug/mL;1:23-94
Immunocytochemistry: 5-20 ug/mL;1:23-94
Optimal working dilutions must be determined by end user
Immunohistochemistry: 5-20 ug/mL;1:23-94
Immunocytochemistry: 5-20 ug/mL;1:23-94
Optimal working dilutions must be determined by end user
Organism Species
Mus musculus (Mouse)
Source
Polyclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant IL3Ra (His167~Lys331 (Accession # P26952)) expressed in E.coli
Cross Reactivity
Mouse
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2056030
Preparation and Storage
Storage: Avoid repeated freeze/thaw cycles.
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Store at 4ºC for frequent use.
Aliquot and store at -20ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition
Western Blot (WB)
(Western Blot: Sample: Recombinant IL3Ra, Mouse.)
Western Blot (WB)
(Western Blot: Sample: Recombinant IL3Ra, Mouse.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
interleukin-3 receptor subunit alpha
NCBI Official Synonym Full Names
interleukin 3 receptor, alpha chain
NCBI Official Symbol
Il3ra
NCBI Official Synonym Symbols
CD123; SUT-1; CDw123
NCBI Protein Information
interleukin-3 receptor subunit alpha
UniProt Protein Name
Interleukin-3 receptor subunit alpha
Protein Family
UniProt Gene Name
Il3ra
UniProt Synonym Gene Names
Sut-1; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
Uniprot Description
IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 14 A1|14 7.08 cM
Cellular Component: integral component of membrane; membrane; plasma membrane
Molecular Function: hematopoietin/interferon-class (D200-domain) cytokine receptor activity; interleukin-3 binding; interleukin-3 receptor activity
Biological Process: cytokine-mediated signaling pathway; monocyte differentiation; regulation of cell growth
Research Articles on IL3Ra
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Product Notes
The IL3Ra il3ra (Catalog #AAA2003792) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 3 Receptor Alpha (IL3Ra) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 3 Receptor Alpha (IL3Ra) can be used in a range of immunoassay formats including, but not limited to, WB; IHC; ICC; IP. Western blotting: 0.5-2 ug/mL;1:230-940 Immunohistochemistry: 5-20 ug/mL;1:23-94 Immunocytochemistry: 5-20 ug/mL;1:23-94 Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the IL3Ra il3ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Interleukin 3 Receptor Alpha (IL3Ra), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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