CD123 / IL3RA recombinant protein

Recombinant Mouse CD123 / IL3RA Protein (ECD, His tag)

Cat. Num. AAA2546778

• Gene Names: Il3ra; CD123; SUT-1; CDw123
• Purity: > 95 % as determined by SDS-PAGE.
• Synonyms: CD123 / IL3RA; Recombinant Mouse CD123 / IL3RA Protein (ECD; His tag); Sut-1; CD123 / IL3RA recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE.
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 396

• Application Notes: The recombinant mouse Il3ra consists 326 amino acids and predicts a molecular mass of 35.9 kDa.
• Predicted N Terminal: Ser 17
• Endotoxin: <1.0 EU per mug protein as determined by the LAL method.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for CD123 / IL3RA recombinant protein

Background: Interleukin-3 receptor subunit alpha, also known as IL-3 receptor subunit alpha, IL-3R-alpha, CD123, and IL3RA, is a single-pass type I membrane protein which belongs to the type I cytokine receptor family and type 5 subfamily. The specific alpha subunit of the interleukin-3 receptor (IL-3Ralpha, CD123) is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. The WSXWS motif of IL3RA appears to be necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding. The box one motif of IL3RA is required for JAK interaction and / or activation. IL3RA represents a unique marker for primitive leukemic stem cells. Targeting of IL3RA may be a promising strategy for the preferential ablation of AML cells. Aberrant IL3RA expression is a good marker for monitoring of minimal residual disease. IL3RA is strongly expressed in various leukemic blasts and leukemic stem cells and seems to be an excellent target for the therapy of leukemias. Recent studies have shown that interleukin-3 receptor alpha (CD123) is highly expressed on leukemia stem cells of patients with acute myeloid leukemia, and is correlated with tumor load and poor prognosis. CD123 was highly expressed in the bone marrow of the patients with myelodysplastic syndrome (MDS), significantly correlated with the proportion of bone marrow blasts, and thus might be the marker of MDS malignant clone. IL3RA is also a useful new marker for distinguishing B-cell disorders with circulating villous lymphocytes as its expression is characteristic of typical hairy cell leukemia (HCL) with high sensitivity and specificity.

Description: A DNA sequence encoding the mouse Il3ra (EDL20630.1) (Met1-Lys331) was expressed with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 148688683
• NCBI GeneID: 16188
• Molecular Weight: 42,017 Da
• NCBI Official Full Name: interleukin 3 receptor, alpha chain, isoform CRA_a
• NCBI Official Synonym Full Names: interleukin 3 receptor, alpha chain
• NCBI Official Symbol: Il3ra
• NCBI Official Synonym Symbols: CD123; SUT-1; CDw123
• NCBI Protein Information: interleukin-3 receptor subunit alpha
• UniProt Protein Name: Interleukin-3 receptor subunit alpha
• UniProt Gene Name: Il3ra
• UniProt Synonym Gene Names: Sut-1; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA

Uniprot Description

IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 14 A1|14 7.08 cM

Cellular Component: membrane

Molecular Function: interleukin-3 binding; interleukin-3 receptor activity

Biological Process: cytokine and chemokine mediated signaling pathway; monocyte differentiation; regulation of cell growth