Rabbit GPR44 Polyclonal Antibody | anti-GPR44 antibody

GPR44 (D113) Polyclonal Antibody

Cat. Num. AAA3003149

• Gene Names: PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: GPR44; Polyclonal Antibody; GPR44 (D113) Polyclonal Antibody; Prostaglandin D2 receptor 2; Chemoattractant receptor-homologous molecule expressed on Th2 cells; G-protein coupled receptor 44; CD_antigen=CD294; CRTH2; DL1R; PTGDR2; DP2; DP-2; anti-GPR44 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: GPR44 (D113) polyclonal antibody detects endogenous levels of GPR44 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 395

• Applicable Applications for anti-GPR44 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 80-127 of Human GPR44.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of GPR44 (D113) pAb at 1:1000 dilutionLane1:SGC7901 whole cell lysate (40ug) Lane2:K562 whole cell lysate (40ug) Lane3:H9C2 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug))

Related Product Information for anti-GPR44 antibody

G protein-coupled receptors (GPCRs), such as GPR44, are integral membrane proteins containing 7 putative transmembrane domains (TMs). These proteins mediate signals to the interior of the cell via activation of heterotrimeric G proteins that in turn activate various effector proteins, ultimately resulting in a physiologic response. GPR44 has recently been found to belong to the prostanoid receptor family and named DP2, with this becoming the official IUPHAR nomenclature. GPR44 has recently been found when activated by elevated level of prostaglandin D2 (PGD2), it could inhibit hair growth. This suggests the PGD2-GPR44 pathway as a potential target for bald treatment. A particularly promising compound for blocking the PGD2-GPR44 pathway is a compound known as TM-30089, which exhibits a "functional insurmountability" of PGD2 expression.

NCBI and Uniprot Product Information

• NCBI GI #: 153791424
• NCBI GeneID: 11251
• NCBI Accession #: NP_004769
• NCBI GenBank Nucleotide #: NP_004769.2
• UniProt Accession #: Q9Y5Y4
• Molecular Weight: ~ 49kDa
• NCBI Official Full Name: prostaglandin D2 receptor 2
• NCBI Official Synonym Full Names: prostaglandin D2 receptor 2
• NCBI Official Symbol: PTGDR2
• NCBI Official Synonym Symbols: DP2; DL1R; CD294; CRTH2; GPR44
• NCBI Protein Information: prostaglandin D2 receptor 2
• UniProt Protein Name: Prostaglandin D2 receptor 2
• UniProt Gene Name: PTGDR2
• UniProt Synonym Gene Names: CRTH2; DL1R; GPR44
• UniProt Entry Name: PD2R2_HUMAN

NCBI Description

This gene encodes a G-protein-coupled receptor that is preferentially expressed in CD4+ effector T helper 2 (Th2) cells. This protein is a prostaglandin D2 receptor that mediates the pro-inflammatory chemotaxis of eosinophils, basophils, and Th2 lymphocytes generated during allergic inflammation. Single nucleotide polymorphisms in the 3' UTR of this gene have been associated with asthma susceptibility.[provided by RefSeq, Mar 2011]

Uniprot Description

GPR44: Receptor for prostaglandin D2 (PGD2). Coupled to the G(i)-protein. Receptor activation may result in pertussis toxin- sensitive decreases in cAMP levels and Ca(2+) mobilization. PI3K signaling is also implicated in mediating PTGDR2 effects. PGD2 induced receptor internalization. CRTH2 internalization can be regulated by diverse kinases such as, PKC, PKA, ADRBK1/GRK2, GPRK5/GRK5 and GRK6. Receptor activation is responsible, at least in part, in immune regulation and allergic/inflammation responses. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass; GPCR, family 1

Chromosomal Location of Human Ortholog: 11q12-q13.3

Cellular Component: neuron projection; integral to plasma membrane; plasma membrane

Molecular Function: G-protein coupled receptor activity; neuropeptide binding; prostaglandin F receptor activity; prostaglandin J receptor activity; prostaglandin D receptor activity

Biological Process: G-protein coupled receptor protein signaling pathway; synaptic transmission; G-protein signaling, coupled to cyclic nucleotide second messenger; neuropeptide signaling pathway; positive regulation of G-protein coupled receptor protein signaling pathway; calcium-mediated signaling; immune response; G-protein signaling, adenylate cyclase inhibiting pathway; chemotaxis