Rabbit Interleukin 2 Polyclonal Antibody | anti-IL2 antibody

Polyclonal Antibody to Interleukin 2 (IL2)

Cat. Num. AAA2027192

• Gene Names: Il2; Il-2
• Reactivity: Mus musculus (Mouse)
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
• Purity: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Synonyms: Interleukin 2; Polyclonal Antibody; Polyclonal Antibody to Interleukin 2 (IL2); anti-IL2 antibody

• Host: Rabbit
• Reactivity: Mus musculus (Mouse)
• Clonality: Polyclonal
• Purity/Purification: Antigen-specific affinity chromatography followed by Protein A affinity chromatography
• Form/Format: Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-IL2 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, Immunoprecipitation (IP)
• Application Notes: Western blotting: 0.5-2ug/mL; Immunohistochemistry: 5-20ug/mL; Immunocytochemistry: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Source: Polyclonal antibody preparation
• Traits: Liquid
• Immunogen: Recombinant IL2 (Val10~Gln169) expressed in E.coli (MBS2031087).
• Preparation and Storage: Storage:; Avoid repeated freeze/thaw cycles.; Store at 4 degree C for frequent use.; Aliquot and store at -20 degree C for 24 months.; ; Stability Test:; thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant IL2, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Uterus Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Skeletal muscle Tissue;Primary Ab: 20ug/ml Rabbit Anti-Mouse IL2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: )

Immunohistochemistry (IHC)

(DAB staining on IHC-P;Samples: Mouse Cerebrum Tissue;Primary Ab: 20ug/ml Rabbit Anti-MouseIL2 AntibodySecond Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: )

NCBI and Uniprot Product Information

• NCBI GI #: 7110653
• NCBI GeneID: 16183
• NCBI Accession #: NP_032392.1
• NCBI GenBank Nucleotide #: NM_008366.3
• UniProt Accession #: P04351; P97945; Q791T3
• NCBI Official Full Name: interleukin-2
• NCBI Official Synonym Full Names: interleukin 2
• NCBI Official Symbol: Il2
• NCBI Official Synonym Symbols: Il-2
• NCBI Protein Information: interleukin-2
• UniProt Protein Name: Interleukin-2
• Protein Family: Interleukin
• UniProt Gene Name: Il2
• UniProt Synonym Gene Names: Il-2; IL-2; TCGF

Uniprot Description

IL2: Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine- activated killer cells, natural killer cells, and glioma cells. A chromosomal aberration involving IL2 is found in a form of T-cell acute lymphoblastic leukemia (T-ALL). Translocation t(4;16)(q26;p13) with involves TNFRSF17. Belongs to the IL-2 family.

Protein type: Cytokine; Oncoprotein; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3 B|3 18.3 cM

Cellular Component: extracellular region; extracellular space

Molecular Function: carbohydrate binding; cytokine activity; glycosphingolipid binding; growth factor activity; interleukin-2 receptor binding; kappa-type opioid receptor binding

Biological Process: adaptive immune response; elevation of cytosolic calcium ion concentration; G-protein coupled receptor signaling pathway; immune response; immune system process; leukocyte activation involved in immune response; negative regulation of B cell apoptotic process; negative regulation of heart contraction; negative regulation of inflammatory response; negative regulation of lymphocyte proliferation; negative regulation of protein phosphorylation; positive regulation of activated T cell proliferation; positive regulation of B cell proliferation; positive regulation of immunoglobulin secretion; positive regulation of interferon-gamma production; positive regulation of interleukin-17 production; positive regulation of isotype switching to IgG isotypes; positive regulation of protein phosphorylation; positive regulation of regulatory T cell differentiation; positive regulation of T cell differentiation; positive regulation of T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of STAT protein; protein kinase C-activating G-protein coupled receptor signaling pathway; regulation of T cell homeostatic proliferation; response to ethanol