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Western Blot (WB)
(Sample: Recombinant LEAP2, Human.)
Rabbit Liver Expressed Antimicrobial Peptide 2 (LEAP2) Polyclonal Antibody | anti-LEAP2 antibody
Polyclonal Antibody to Liver Expressed Antimicrobial Peptide 2 (LEAP2)
Gene Names
LEAP2; LEAP-2
Reactivity
Homo sapiens (Human)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Liver Expressed Antimicrobial Peptide 2 (LEAP2); Polyclonal Antibody; Polyclonal Antibody to Liver Expressed Antimicrobial Peptide 2 (LEAP2); anti-LEAP2 antibody
Host
Rabbit
Reactivity
Homo sapiens (Human)
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Concentration
0.26mg/ml (varies by lot)
Applicable Applications for anti-LEAP2 antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
I mmunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
I mmunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Mouse; Rat; Pig
Immunogen
Recombinant LEAP2 (Ser23~Gln76) expressed in E.coli (MBS2009421).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Sample: Recombinant LEAP2, Human.)
Western Blot (WB)
(Sample: Recombinant LEAP2, Human.)
Western Blot (WB)
(Sample:Lane 1: Porcine Liver TissueLane 2: Porcine Skeletal Muscle Tissue.)
Western Blot (WB)
(Sample:Lane 1: Porcine Liver TissueLane 2: Porcine Skeletal Muscle Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Prostate Gland Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Prostate Gland Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Rectum Tissue;Primary Ab: 10ug/mL Rabbit Anti-Human LEAP2 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: )
Immunohistochemistry (IHC)
(DAB staining on IHC-P;Samples: Human Rectum Tissue;Primary Ab: 10ug/mL Rabbit Anti-Human LEAP2 Antibody Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: )
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
liver-expressed antimicrobial peptide 2
NCBI Official Synonym Full Names
liver enriched antimicrobial peptide 2
NCBI Official Symbol
LEAP2
NCBI Official Synonym Symbols
LEAP-2
NCBI Protein Information
liver-expressed antimicrobial peptide 2
UniProt Protein Name
Liver-expressed antimicrobial peptide 2
Protein Family
UniProt Gene Name
LEAP2
UniProt Synonym Gene Names
LEAP-2
NCBI Description
This gene encodes a cysteine-rich cationic antimicrobial peptide that is expressed predominantly in the liver. The mature peptide has activity against gram-positive bacteria and yeasts. [provided by RefSeq, Sep 2014]
Uniprot Description
LEAP2: Has an antimicrobial activity. Belongs to the LEAP2 family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 5q31.1
Cellular Component: extracellular region
Biological Process: antimicrobial humoral response; defense response to bacterium
Research Articles on LEAP2
Similar Products
Product Notes
The LEAP2 leap2 (Catalog #AAA2001931) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Liver Expressed Antimicrobial Peptide 2 (LEAP2) reacts with Homo sapiens (Human) and may cross-react with other species as described in the data sheet. AAA Biotech's Liver Expressed Antimicrobial Peptide 2 (LEAP2) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL I mmunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the LEAP2 leap2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Liver Expressed Antimicrobial Peptide 2 (LEAP2), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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