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Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using NNMT Antibody. The lane on the left was treated with blocking peptide.)

Rabbit NNMT Polyclonal Antibody | anti-NNMT antibody

NNMT Antibody

Reactivity
Human, Mouse, Rat
Predicted: Pig, Dog
Applications
Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Synonyms
NNMT; Polyclonal Antibody; NNMT Antibody; EC 2.1.1.1; Nicotinamide N methyltransferase; Nicotinamide N-methyltransferase; NNMT_HUMAN; anti-NNMT antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted: Pig, Dog
Clonality
Polyclonal
Isotype
IgG
Specificity
NNMT Antibody detects endogenous levels of NNMT.
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
Form/Format
Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
264
Applicable Applications for anti-NNMT antibody
Western Blot (WB)
Application Notes
WB: 1:500-1:2000
Immunogen
A synthesized peptide derived from human NNMT.
Tissue Specificity
Predominantly expressed in the liver. A lower expression is seen in the kidney, lung, skeletal muscle, placenta and heart. Not detected in the brain or pancreas.
Fragment
Fab Fragment
Conjugate
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from HepG2 cells, using NNMT Antibody. The lane on the left was treated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from HepG2 cells, using NNMT Antibody. The lane on the left was treated with blocking peptide.)
Related Product Information for anti-NNMT antibody
Description: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds.

Subunit Structure: Monomer.

Similarity: Belongs to the class I-like SAM-binding methyltransferase superfamily. NNMT/PNMT/TEMT family.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 30 kDa
Predicted: 30 kDa
NCBI Official Full Name
nicotinamide N-methyltransferase
NCBI Official Synonym Full Names
nicotinamide N-methyltransferase
NCBI Official Symbol
NNMT
NCBI Protein Information
nicotinamide N-methyltransferase
UniProt Protein Name
Nicotinamide N-methyltransferase
UniProt Gene Name
NNMT
UniProt Entry Name
NNMT_HUMAN

NCBI Description

N-methylation is one method by which drug and other xenobiotic compounds are metabolized by the liver. This gene encodes the protein responsible for this enzymatic activity which uses S-adenosyl methionine as the methyl donor. [provided by RefSeq, Jul 2008]

Uniprot Description

NNMT: Catalyzes the N-methylation of nicotinamide and other pyridines to form pyridinium ions. This activity is important for biotransformation of many drugs and xenobiotic compounds. Belongs to the NNMT/PNMT/TEMT family.

Protein type: Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; EC 2.1.1.1; Methyltransferase

Chromosomal Location of Human Ortholog: 11q23.1

Cellular Component: cytosol

Molecular Function: nicotinamide N-methyltransferase activity

Biological Process: response to drug; methylation; organ regeneration; xenobiotic metabolic process; response to organic nitrogen

Research Articles on NNMT

Similar Products

Product Notes

The NNMT nnmt (Catalog #AAA9611940) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NNMT Antibody reacts with Human, Mouse, Rat Predicted: Pig, Dog and may cross-react with other species as described in the data sheet. AAA Biotech's NNMT can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500-1:2000. Researchers should empirically determine the suitability of the NNMT nnmt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NNMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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