Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Rabbit anti-Mouse Interleukin 1 Beta (IL1b) Polyclonal Antibody | anti-IL1b antibody
Polyclonal Antibody to Interleukin 1 Beta (IL1b)
MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-VPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF LGNNSGQDII DFTMESVSS
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
Immunohistochemistry (IHC)
(DABstainingonIHC-P.Samples:MouseTissue))
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1. The encoded protein plays a role in thymocyte proliferation and is involved in the inflammatory response. [provided by RefSeq, Aug 2015]
Uniprot Description
IL1B: Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. Monomer. Belongs to the IL-1 family.
Protein type: Cytokine
Chromosomal Location of Human Ortholog: 2 F1|2 62.97 cM
Cellular Component: cytoplasm; cytoplasmic vesicle; extracellular region; extracellular space; lysosome; secretory granule; vesicle
Molecular Function: cytokine activity; interleukin-1 receptor binding; protein domain specific binding
Biological Process: activation of MAPK activity; cellular response to organic cyclic compound; cellular response to organic substance; cytokine-mediated signaling pathway; elevation of cytosolic calcium ion concentration; fever; germ cell programmed cell death; hyaluronan biosynthetic process; immune response; inflammatory response; interleukin-1 beta production; interleukin-6 production; leukocyte migration; lipopolysaccharide-mediated signaling pathway; MAPK cascade; memory; negative regulation of branching morphogenesis of a nerve; negative regulation of cell proliferation; negative regulation of extrinsic apoptotic signaling pathway in absence of ligand; negative regulation of gene expression; negative regulation of glucose transport; negative regulation of glutamate secretion; negative regulation of insulin receptor signaling pathway; negative regulation of lipid catabolic process; negative regulation of lipid metabolic process; negative regulation of MAP kinase activity; negative regulation of neural precursor cell proliferation; negative regulation of neurogenesis; negative regulation of neuron differentiation; negative regulation of synaptic transmission; negative regulation of transcription from RNA polymerase II promoter; neutrophil chemotaxis; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of astrocyte differentiation; positive regulation of cell death; positive regulation of cell division; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of chemokine biosynthetic process; positive regulation of epithelial to mesenchymal transition; positive regulation of ERK1 and ERK2 cascade; positive regulation of fever; positive regulation of gene expression; positive regulation of glial cell differentiation; positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of heterotypic cell-cell adhesion; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of immature T cell proliferation in the thymus; positive regulation of interferon-gamma production; positive regulation of interleukin-2 biosynthetic process; positive regulation of interleukin-6 biosynthetic process; positive regulation of interleukin-6 production; positive regulation of interleukin-6 secretion; positive regulation of interleukin-8 production; positive regulation of JNK cascade; positive regulation of JUN kinase activity; positive regulation of lipid catabolic process; positive regulation of membrane protein ectodomain proteolysis; positive regulation of mitotic nuclear division; positive regulation of monocyte chemotactic protein-1 production; positive regulation of neuron apoptotic process; positive regulation of neutrophil chemotaxis; positive regulation of NF-kappaB import into nucleus; positive regulation of NF-kappaB transcription factor activity; positive regulation of nitric oxide biosynthetic process; positive regulation of p38MAPK cascade; positive regulation of phagocytosis; positive regulation of prostaglandin secretion; positive regulation of protein phosphorylation; positive regulation of stress-activated MAPK cascade; positive regulation of T cell proliferation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-templated; positive regulation of vascular endothelial growth factor production; protein kinase B signaling; regulation of defense response to virus by host; regulation of ERK1 and ERK2 cascade; regulation of I-kappaB kinase/NF-kappaB cascade; regulation of insulin secretion; regulation of nitric-oxide synthase activity; response to ATP; response to carbohydrate stimulus; response to lipopolysaccharide; sequestering of triacylglycerol; social behavior
Research Articles on IL1b
Similar Products
Product Notes
The IL1b il1b (Catalog #AAA2006218) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Interleukin 1 Beta (IL1b) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Interleukin 1 Beta (IL1b) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IL1b il1b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MRNKKFEL GLEFPNLPYY IDGDVKLTQS MAIIRYIADK HNMLGGCPKE RAEISMLEGA VLDIRYGVSR IAYSKDFETL KVDFLSKLPE MLKMFEDRLC HKTYLNGDHV THPDFMLYDA LDVVLYMDPM CLDAFPKLVC FKKRIEAIPQ IDKYLKSSKY IAWPLQGWQA TFGGGDHPPK SDGSTSGSGH HHHHHSAGLV PRGSTAIGMK ETAAAKFERQ HMDSPDLGTL EVLFQGPLGS EF-VPI RQLHYRLRDE QQKSLVLSDP YELKALHLNG QNINQQVIFS MSFVQGEPSN DKIPVALGLK GKNLYLSCVM KDGTPTLQLE SVDPKQYPKK KMEKRFVFNK IEVKSKVEFE SAEFPNWYIS TSQAEHKPVF LGNNSGQDII DFTMESVSS. It is sometimes possible for the material contained within the vial of "Interleukin 1 Beta (IL1b), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
