IGF-I R/IGF1R Recombinant Protein | IGF-IR/IGF1R recombinant protein

Recombinant Human IGF-I R/IGF1R (C-6His)

Cat. Num. AAA2569197

• Gene Names: IGF1R; IGFR; CD221; IGFIR; JTK13
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: IGF-I R/IGF1R; Recombinant Human IGF-I R/IGF1R (C-6His); CD221 antigen; CD221; IGF1R; IGF-1R; IGF-I R; IGF-I receptor; IGFIR; IGF-IR; IGFR; insulin-like growth factor 1 receptor; JTK13; IGF-IR/IGF1R recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, 1mM EDTA , 0.5% Tween-20, 5% Trehalose, pH7.4.
• Sequence: Glu31-Asn932

• Species: Human
• Tag: C-6His
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Insulin-like Growth Factor 1 Receptor is produced by our Mammalian expression system and the target gene encoding Glu31-Asn932 is expressed with a 6His tag at the C-terminus.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Related Product Information for IGF-IR/IGF1R recombinant protein

The insulin-like growth factor-1 receptor (IGF1R) is a transmembrane tyrosine kinase involved in several biological processes including cell proliferation, differentiation, DNA repair, and cell survival. This a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits, and among which, the alpha subunit is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. The IGF-I receptor is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and II have been shown to be mediated via IGF-I R. IGF1R is an important signaling molecule in cancer cells and plays an essential role in the establishment and maintenance of the transformed phenotype. Inhibition of IGF1R signaling thus appears to be a promising strategy to interfere with the growth and survival of cancer cells, is now an attractive anti-cancer treatment target.

Product Categories/Family for IGF-IR/IGF1R recombinant protein

Signal Transduction; Cardiovascular; Neuroscience; Cancer; Developmental Biology; metabolism

NCBI and Uniprot Product Information

• NCBI GI #: 4557665
• NCBI GeneID: 3480
• NCBI Accession #: NP_000866.1
• NCBI GenBank Nucleotide #: NM_000875.3
• UniProt Accession #: P08069; Q14CV2; Q9UCC0; B1B5Y2
• Molecular Weight: 154,793 Da
• NCBI Official Full Name: insulin-like growth factor 1 receptor
• NCBI Official Synonym Full Names: insulin-like growth factor 1 receptor
• NCBI Official Symbol: IGF1R
• NCBI Official Synonym Symbols: IGFR; CD221; IGFIR; JTK13
• NCBI Protein Information: insulin-like growth factor 1 receptor; IGF-I receptor; soluble IGF1R variant 1; soluble IGF1R variant 2; insulin-like growth factor I receptor
• UniProt Protein Name: Insulin-like growth factor 1 receptor
• UniProt Gene Name: IGF1R
• UniProt Synonym Gene Names: IGF-I receptor
• UniProt Entry Name: IGF1R_HUMAN

NCBI Description

This receptor binds insulin-like growth factor with a high affinity. It has tyrosine kinase activity. The insulin-like growth factor I receptor plays a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. It is highly overexpressed in most malignant tissues where it functions as an anti-apoptotic agent by enhancing cell survival. [provided by RefSeq, Jul 2008]

Uniprot Description

IGF1R: a receptor tyrosine kinase that binds insulin-like growth factor 1 (IGF1) with a high affinity and IGF2 with a lower affinity. Functions as an anti-apoptotic agent by enhancing cell survival. Can play a critical role in transformation events. Cleavage of the precursor generates alpha and beta subunits. Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chain carries the kinase domain. Interacts with PIK3R1 and with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. Mutated in rare cases of pre- and post-natal growth retardation. One SNP associated with increased human longevity. Increased expression of IGF1R and other pathway members associated with progression and malignancy in a range of cancers. Inhibitors: AG1024, AEW541.

Protein type: Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; Protein kinase, TK; EC 2.7.10.1; TK group; InsR family

Chromosomal Location of Human Ortholog: 15q26.3

Cellular Component: neuron projection; intracellular membrane-bound organelle; membrane; integral to plasma membrane; plasma membrane; caveola; receptor complex

Molecular Function: insulin binding; identical protein binding; insulin-like growth factor binding; protein binding; insulin-like growth factor I binding; protein-tyrosine kinase activity; insulin receptor substrate binding; G-protein alpha-subunit binding; phosphoinositide 3-kinase binding; insulin-like growth factor receptor activity; insulin receptor binding; ATP binding

Biological Process: phosphoinositide 3-kinase cascade; epidermis development; inactivation of MAPKK activity; protein heterooligomerization; protein amino acid autophosphorylation; exocrine pancreas development; signal transduction; regulation of JNK cascade; response to vitamin E; mammary gland development; positive regulation of MAPKKK cascade; male sex determination; positive regulation of cell proliferation; protein tetramerization; positive regulation of cytokinesis; phosphoinositide-mediated signaling; negative regulation of protein kinase B signaling cascade; positive regulation of mitosis; negative regulation of transcription factor activity; positive regulation of protein kinase B signaling cascade; axonogenesis; insulin-like growth factor receptor signaling pathway; establishment of cell polarity; insulin receptor signaling pathway; immune response; brain development; positive regulation of DNA replication; positive regulation of cell migration; negative regulation of apoptosis

Disease: Insulin-like Growth Factor I, Resistance To