Goat anti-Human Insulin-Like Growth Factor Binding Protein-Like 1 Polyclonal Antibody | anti-IGFBPL1 antibody

Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBP-L1)

Cat. Num. AAA610772

• Gene Names: IGFBPL1; IGFBP-RP4; bA113O24.1
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by human IGFBP-L1 affinity chromatography.
• Synonyms: Insulin-Like Growth Factor Binding Protein-Like 1; Polyclonal Antibody; Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBP-L1); Anti -Insulin-Like Growth Factor Binding Protein-Like 1 (IGFBP-L1); anti-IGFBPL1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Selected for its ability to recognize human IGFBP-L1 in direct ELISAs and Western Blots.
• Purity/Purification: Affinity Purified; Purified by human IGFBP-L1 affinity chromatography.
• Form/Format: Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.5ml is used, the concentration will be 0.2mg/ml.

• Applicable Applications for anti-IGFBPL1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human IGFBP-L1. The detection limit for rhIGFBP-L1 is approximately 2 ng/lane under non-reducing and reducing conditions. In this format, this antibody shows approximately 15% cross-reactivity with rmIGFBP-L1 and less than 1% cross-reactivity with rhIGFBP-1, -2, -3, -4, -5, -6, -7, and rmIGFBP-rP10., Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human IGFBP-L1. The detection limit for rhIGFBP-L1 is approximately 0.5 ng/well., Optimal dilutions should be determined by each laboratory for each application.
• Immunogen: NS0-derived rhIGFBP-L1
• Preparation and Storage: Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-IGFBPL1 antibody

IGFBP-L1 is a secreted 38 kD protein that shares 43% amino acid sequence identity with IGFBP-7 (IGFBP-rP1, Mac25). Although it was also referred to as IGFBP-rP4 in one publication, it is distinct from Cyr 61, a CCN family protein also named IGFBP-rP4 at one time. IGFBP-L1 contains an N-terminal IGFBP motif, a Kazal-type serine protease inhibitor region and a C-terminal Ig-like domain. Human and mouse mature IGFBP-L1 share 76% amino acid sequence homology.

Product Categories/Family for anti-IGFBPL1 antibody

Antibodies; Abs to Growth Factors; Cytokines

NCBI and Uniprot Product Information

• NCBI GI #: 56090548
• NCBI GeneID: 347252
• NCBI Accession #: NP_001007564.1
• NCBI GenBank Nucleotide #: NM_001007563.1
• UniProt Accession #: Q8WX77
• Molecular Weight: 29,005 Da
• NCBI Official Full Name: insulin-like growth factor-binding protein-like 1
• NCBI Official Synonym Full Names: insulin-like growth factor binding protein-like 1
• NCBI Official Symbol: IGFBPL1
• NCBI Official Synonym Symbols: IGFBP-RP4; bA113O24.1
• NCBI Protein Information: insulin-like growth factor-binding protein-like 1; OTTHUMP00000021398; IGFBP-related protein 10; insulin-like growth factor-binding-related protein 4; insulin-like growth factor binding protein related protein 4
• UniProt Protein Name: Insulin-like growth factor-binding protein-like 1
• Protein Family: Insulin-like growth factor-binding protein
• UniProt Gene Name: IGFBPL1
• UniProt Synonym Gene Names: IGFBPRP4
• UniProt Entry Name: IBPL1_HUMAN

Uniprot Description

IGFBPL1: IGF-binding proteins prolong the half-life of IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs in cell culture. They alter the interaction of IGFs with their cell surface receptors. May be a putative tumor suppressor protein.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 9p13.1

Cellular Component: extracellular region

Molecular Function: insulin-like growth factor binding

Biological Process: regulation of cell growth