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Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Rabbit anti-Rat Insulin Like Growth Factor Binding Protein 5 (IGFBP5) Polyclonal Antibody | anti-IGFBP5 antibody

Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 5 (IGFBP5)

Gene Names
Igfbp5; IGF-BP5
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Insulin Like Growth Factor Binding Protein 5 (IGFBP5); Polyclonal Antibody; Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 5 (IGFBP5); anti-IGFBP5 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against IGFBP5. It has been selected for its ability to recognize IGFBP5 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with a His-tag and its sequence is listed below.
MGHHHHHHSGS-HCEPCD EKALSMCPPS PLGCELVKEP GCGCCMTCAL AEGQSCGVYT ERCAQGLRCL PRQDEEKPLH ALLHGRGVCL NEKSYGEQTK IERDSREHEE PTTSEMAEET YSPKVFRPKH TRISELKAEA VKKDRRKKLT QSKFVGGAEN TAHPRVIPAP EMRQESDQGP CRRHMEASLQ EFKASPRMVP RAVYLPNCDR KGFYKRKQCK PSRGRKRGIC WCVDKYGMKL PGMEYVDGDF QCHAFDSSNV E
Sequence Length
272
Applicable Applications for anti-IGFBP5 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant IGFBP5 (His25~Glu271) expressed in E.coli.
Cross Reactivity
Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2067462
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Western Blot (WB) (Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on fromalin fixed paraffin-embedded kidney tissue))

Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded kidney tissue))

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,298 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 5
NCBI Official Synonym Full Names
insulin-like growth factor binding protein 5
NCBI Official Symbol
Igfbp5
NCBI Official Synonym Symbols
IGF-BP5
NCBI Protein Information
insulin-like growth factor-binding protein 5
UniProt Protein Name
Insulin-like growth factor-binding protein 5
UniProt Gene Name
Igfbp5
UniProt Synonym Gene Names
Igfbp-5; IBP-5; IGF-binding protein 5; IGFBP-5

NCBI Description

may be involved in ovarian folliculogenesis and may play a modulatory role in type I fiber-dominated muscles [RGD, Feb 2006]

Uniprot Description

IGFBP5: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

Protein type: Cell cycle regulation; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q33

Cellular Component: extracellular region; extracellular space

Molecular Function: fibronectin binding; insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding

Biological Process: aging; alveolus development; female pregnancy; glucose homeostasis; glucose metabolic process; hair follicle morphogenesis; mammary gland involution; negative regulation of cell migration; negative regulation of growth; negative regulation of insulin-like growth factor receptor signaling pathway; negative regulation of osteoblast differentiation; negative regulation of smooth muscle cell migration; negative regulation of smooth muscle cell proliferation; negative regulation of translation; osteoblast differentiation; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of protein kinase B signaling; regulation of cell growth; regulation of growth; regulation of insulin-like growth factor receptor signaling pathway; striated muscle cell differentiation

Research Articles on IGFBP5

Similar Products

Product Notes

The IGFBP5 igfbp5 (Catalog #AAA2002469) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Insulin Like Growth Factor Binding Protein 5 (IGFBP5) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Insulin Like Growth Factor Binding Protein 5 (IGFBP5) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the IGFBP5 igfbp5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with a His-tag and its sequence is listed below. MGHHHHHHSG S-HCEPCD EKALSMCPPS PLGCELVKEP GCGCCMTCAL AEGQSCGVYT ERCAQGLRCL PRQDEEKPLH ALLHGRGVCL NEKSYGEQTK IERDSREHEE PTTSEMAEET YSPKVFRPKH TRISELKAEA VKKDRRKKLT QSKFVGGAEN TAHPRVIPAP EMRQESDQGP CRRHMEASLQ EFKASPRMVP RAVYLPNCDR KGFYKRKQCK PSRGRKRGIC WCVDKYGMKL PGMEYVDGDF QCHAFDSSNV E. It is sometimes possible for the material contained within the vial of "Insulin Like Growth Factor Binding Protein 5 (IGFBP5), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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