Rabbit anti-Human, Mouse INSM1 Polyclonal Antibody | anti-INSM1 antibody

INSM1 Antibody

Cat. Num. AAA9630873

• Gene Names: INSM1; IA1; IA-1
• Reactivity: Human, Mouse
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: INSM1; Polyclonal Antibody; INSM1 Antibody; IA1; Insulinoma associated protein 1; Zinc finger protein IA1; anti-INSM1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: INSM1 Antibody detects endogenous levels of INSM1.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-INSM1 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human INSM1.
• Conjugation: Unconjugated
• Similarity: The C-terminal region is necessary for NEUROD1 promoter DNA-binding and transcriptional repressor activity. Belongs to the INSM1 family.
• Subunit Structure: Interacts (via the SNAG domain) with HDAC1. Interacts (via the SNAG domain) with HDAC2 (By similarity). Interacts (via the SNAG domain) with KDM1A. Interacts (via the SNAG domain) with RCOR1. Interacts with SORBS1 (By similarity). Interacts (via the N-terminal region) with CCND1 (via cyclin N-terminal domain); the interaction competes with the binding of CCND1 to CDK4 during cell cycle progression and increases its transcriptional repressor activity. Interacts with HDAC3; the interaction increases its transcriptional repressor activity.
• Tissue Specificity: Expressed in pancreatic duct cells. Expressed in several tumor cell lines of neuroendocrine origin including pheochromocytoma, medullary thyroid carcinoma, insulinoma, medulloblastoma, retinoblastoma, pheochromacytoma, medullary thyroid carcinoma and small cell lung carcinoma.
• Function: Sequence-specific DNA-binding transcriptional regulator that plays a key role in neurogenesis and neuroendocrine cell differentiation during embryonic and/or fetal development. Binds to the consensus sequence 5'-[TG][TC][TC][TT][GA]GGG[CG]A-3' in target promoters. Acts as a transcriptional repressor of NEUROD1 and INS expression via its interaction with cyclin CCND1 in a cell cycle-independent manner. Negatively regulates skeletal muscle-specific gene expression in endocrine cells of the pituitary by inhibiting the Notch signaling pathway. Represses target gene transcription by recruiting chromatin-modifying factors, such as HDAC1, HDAC2, HDAC3, KDM1A and RCOR1 histone deacetylases. Binds to its own promoter, suggesting autoregulation as a self-control feedback mechanism. Competes with histone H3 for the same binding site on the histone demethylase complex formed by KDM1A and RCOR1, and thereby inhibits demethylation of histone H3 at 'Lys-4'. Promotes the generation and expansion of neuronal basal progenitor cells in the developing neocortex. Involved in the differentiation of endocrine cells of the developing anterior pituitary gland, of the pancreas and intestine, and of sympatho-adrenal cells in the peripheral nervous system. Promotes cell cycle signaling arrest and inhibition of cellular proliferation.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-INSM1 antibody

Nucleus; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 4504713
• NCBI GeneID: 3642
• NCBI Accession #: NP_002187
• NCBI GenBank Nucleotide #: NM_002196.2
• UniProt Accession #: Q01101
• Molecular Weight: 52,923 Da
• NCBI Official Full Name: insulinoma-associated protein 1
• NCBI Official Synonym Full Names: insulinoma-associated 1
• NCBI Official Symbol: INSM1
• NCBI Official Synonym Symbols: IA1; IA-1
• NCBI Protein Information: insulinoma-associated protein 1; zinc finger protein IA-1
• UniProt Protein Name: Insulinoma-associated protein 1
• Protein Family: Insulinoma-associated protein
• UniProt Gene Name: INSM1
• UniProt Synonym Gene Names: IA1
• UniProt Entry Name: INSM1_HUMAN

NCBI Description

Insulinoma-associated 1 (INSM1) gene is intronless and encodes a protein containing both a zinc finger DNA-binding domain and a putative prohormone domain. This gene is a sensitive marker for neuroendocrine differentiation of human lung tumors. [provided by RefSeq, Jul 2008]

Uniprot Description

INSM1: May be associated with the transformation of neuroendocrine cells.

Protein type: C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 20p11.2

Cellular Component: nucleoplasm; transcriptional repressor complex; nucleus

Molecular Function: cyclin binding; chromatin DNA binding; histone deacetylase binding; metal ion binding; transcription factor activity

Biological Process: norepinephrine biosynthetic process; negative regulation of cell proliferation; transcription, DNA-dependent; negative regulation of protein amino acid phosphorylation; regulation of gene expression; regulation of protein complex assembly; negative regulation of transcription from RNA polymerase II promoter; cell cycle; positive regulation of cell differentiation; endocrine pancreas development