Rabbit anti-Human SCLT1 Polyclonal Antibody | anti-SCLT1 antibody

SCLT1, CT (SCLT1, SAP1, Sodium channel and clathrin linker 1, Sodium channel-associated protein 1) (APC)

Cat. Num. AAA6345306

• Gene Names: SCLT1; CAP1A; CAP-1A
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: SCLT1; Polyclonal Antibody; CT (SCLT1; SAP1; Sodium channel and clathrin linker 1; Sodium channel-associated protein 1) (APC); Sodium channel-associated protein 1; anti-SCLT1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-SCLT1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: SCLT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 576-605 amino acids from the C-terminal region of human SCLT1.
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(SCLT1 Antibody (C-term) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the SCLT1 antibody detected the SCLT1 protein (arrow).)

Immunohistochemistry (IHC)

(SCLT1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SCLT1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-SCLT1 antibody

SCLT1 acts as a linker protein between the voltage-gated sodium channel Na(v)1.8 (SCN10A; MIM 604427) and clathrin (see CLTC, MIM 118955) (Liu et al., 2005 [PubMed 15797711]).[supplied by OMIM].

Product Categories/Family for anti-SCLT1 antibody

Antibodies; Clathrin

References

Liu, C., et al. Mol. Cell. Neurosci. 28(4):636-649(2005). Tomarev, S.I., et al. Invest. Ophthalmol. Vis. Sci. 44(6):2588-2596(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 153791839
• NCBI GeneID: 132320
• NCBI Accession #: NP_653244.2
• NCBI GenBank Nucleotide #: NM_144643.3
• UniProt Accession #: Q96NL6; Q0VAH2; Q6P2M4; A4QN04
• Molecular Weight: 80,910 Da
• NCBI Official Full Name: sodium channel and clathrin linker 1 isoform 1
• NCBI Official Synonym Full Names: sodium channel and clathrin linker 1
• NCBI Official Symbol: SCLT1
• NCBI Official Synonym Symbols: CAP1A; CAP-1A
• NCBI Protein Information: sodium channel and clathrin linker 1; sodium channel-associated protein 1
• UniProt Protein Name: Sodium channel and clathrin linker 1
• Protein Family: Sodium channel and clathrin linker
• UniProt Gene Name: SCLT1
• UniProt Synonym Gene Names: SAP1
• UniProt Entry Name: SCLT1_HUMAN

Uniprot Description

SCLT1: Adapter protein that links SCN10A to clathrin. Regulates SCN10A channel activity, possibly by promoting channel internalization. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Unknown function

Chromosomal Location of Human Ortholog: 4q28.2

Cellular Component: centriole; centrosome; cytosol

Molecular Function: protein C-terminus binding; clathrin binding; sodium channel regulator activity

Biological Process: clustering of voltage-gated sodium channels; organelle organization and biogenesis; cilium biogenesis