Rabbit anti-Mouse Inhibitory Subunit Of NF Kappa B Beta (IkBb) Polyclonal Antibody | anti-IkBb antibody

Polyclonal Antibody to Inhibitory Subunit Of NF Kappa B Beta (IkBb)

Cat. Num. AAA2002054

• Gene Names: Nfkbib; IkB; IkBb; ikB-B; IKB-beta; IKappaBbeta; NF-kappa-BIB; I-kappa-B-beta; I(Kappa)B(beta)
• Reactivity: Mouse
• Applications: Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
• Purity: Affinity Chromatography
• Synonyms: Inhibitory Subunit Of NF Kappa B Beta (IkBb); Polyclonal Antibody; Polyclonal Antibody to Inhibitory Subunit Of NF Kappa B Beta (IkBb); anti-IkBb antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against IkBb. It has been selected for its ability to recognize IkBb in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF-GAGV LVAERGGHTA LHLACRVRAH TCACVLLQPR PSHPRDASDT YLTQSQDCTP DTSHAPAAVD SQPNPENEEE PRDEDWRLQL EAENYDGHTP LHVAVIHKDA EMVRLLRDAG ADLNKPEPTC GRTPLHLAVE AQAASVLELL LKAGADPTAR MYGGRTPLGS ALLRPNPILA RLLRAHGAPE PEDEDDKLSP CSSSGSDSDS DNRDEGDEYD DIVVHSGRSQ NRQPP
• Sequence Length: 756

• Applicable Applications for anti-IkBb antibody: Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
• Application Notes: Western blotting: 1:100-400; Immunocytochemistry in formalin fixed cells: 1:100-500; Immunohistochemistry in formalin fixed frozen section: 1:100-500; Immunohistochemistry in paraffin section: 1:50-200; Enzyme-linked Immunosorbent Assay: 1:100-200
• Immunogen: Recombinant IkBb (Gly117~Pro345) expressed in E.coli.
• Cross Reactivity: Human
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2059832
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Human Hela cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse IkBb Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )

Western Blot (WB)

(Western Blot: Sample: Recombinant protein.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P. Samples: Mouse Tissue))

NCBI and Uniprot Product Information

• NCBI GI #: 815890832
• NCBI GeneID: 18036
• NCBI Accession #: NP_001293151.1
• NCBI GenBank Nucleotide #: NM_001306222.1
• UniProt Accession #: Q60778; Q564F1; Q9D6L5
• Molecular Weight: 37,965 Da
• NCBI Official Full Name: NF-kappa-B inhibitor beta
• NCBI Official Synonym Full Names: nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, beta
• NCBI Official Symbol: Nfkbib
• NCBI Official Synonym Symbols: IkB; IkBb; ikB-B; IKB-beta; IKappaBbeta; NF-kappa-BIB; I-kappa-B-beta; I(Kappa)B(beta)
• NCBI Protein Information: NF-kappa-B inhibitor beta
• UniProt Protein Name: NF-kappa-B inhibitor beta
• UniProt Gene Name: Nfkbib
• UniProt Synonym Gene Names: Ikbb; NF-kappa-BIB; IkB-B; IkB-beta; IkappaBbeta

NCBI Description

This gene encodes an inhibitor of nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappaB). The encoded protein prevents NF-kappaB-mediated transcription activation by sequestering it in the cytosol. In response to signals that induce NF-kappaB, such as cytokines and growth factors, the encoded protein undergoes phosphorylation, triggering its rapid ubiquitination and proteasomal degradation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]

Uniprot Description

Inhibits NF-kappa-B by complexing with and trapping it in the cytoplasm. However, the unphosphorylated form resynthesized after cell stimulation is able to bind NF-kappa-B allowing its transport to the nucleus and protecting it to further NFKBIA-dependent inactivation. Association with inhibitor kappa B-interacting NKIRAS1 and NKIRAS2 prevent its phosphorylation rendering it more resistant to degradation, explaining its slower degradation.