Rabbit anti-Human INHA Polyclonal Antibody | anti-INHA antibody

INHA, NT (Inhibin alpha Chain) (PE)

Cat. Num. AAA6481730

• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: INHA; Polyclonal Antibody; NT (Inhibin alpha Chain) (PE); anti-INHA antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human INHA.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-INHA antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human INHA (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of NHA Antibody (N-term) in mouse testis tissue lysates (35ug/lane).NHA (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of INHA(arrow) using rabbit polyclonal INHA Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the INHA gene (Lane 2) (Origene Technologies).)

Immunohistochemistry (IHC)

(INHA Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the INHA Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of INHA Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of INHA Antibody (N-term) with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Product Categories/Family for anti-INHA antibody

Antibodies; Hormones; Steroids

References

1. Balanathan, P., Br. J. Cancer 100(11), 1784-1793 (2009). 2. Mason, A.J., Mol. Endocrinol. 10(9), 1055-1065 (1996).

NCBI and Uniprot Product Information

• NCBI GI #: 4504697
• NCBI GeneID: 3623
• NCBI Accession #: NP_002182
• NCBI GenBank Nucleotide #: NM_002191.3
• UniProt Accession #: P05111; A8K8H5
• Molecular Weight: 17.0kDa (155aa)
• NCBI Official Full Name: inhibin alpha chain preproprotein
• NCBI Official Synonym Full Names: inhibin alpha subunit
• NCBI Official Symbol: INHA
• NCBI Protein Information: inhibin alpha chain
• UniProt Protein Name: Inhibin alpha chain
• Protein Family: Inhibin
• UniProt Gene Name: INHA
• UniProt Entry Name: INHA_HUMAN

NCBI Description

This gene encodes a member of the TGF-beta (transforming growth factor-beta) superfamily of proteins. The encoded preproprotein is proteolytically processed to generate multiple peptide products, including the alpha subunit of the inhibin A and B protein complexes. These complexes negatively regulate follicle stimulating hormone secretion from the pituitary gland. Inhibins have also been implicated in regulating numerous cellular processes including cell proliferation, apoptosis, immune response and hormone secretion. Mutations in this gene may be associated with male infertility and premature ovarian failure in female human patients. [provided by RefSeq, Aug 2016]

Uniprot Description

INHA: Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. Belongs to the TGF-beta family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: photoreceptor inner segment; photoreceptor outer segment; cell soma; extracellular region

Molecular Function: protein binding; growth factor activity; protein heterodimerization activity; hormone activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: negative regulation of B cell differentiation; hemoglobin biosynthetic process; nervous system development; regulation of cell cycle; male gonad development; signal transduction; regulation of cell proliferation; negative regulation of interferon-gamma biosynthetic process; negative regulation of cell cycle; negative regulation of follicle-stimulating hormone secretion; response to external stimulus; regulation of apoptosis; cell surface receptor linked signal transduction; negative regulation of macrophage differentiation; ovarian follicle development; cell-cell signaling; regulation of MAPKKK cascade; erythrocyte differentiation; negative regulation of phosphorylation; positive regulation of follicle-stimulating hormone secretion; cell differentiation; cell cycle arrest; skeletal development; cell development