Goat anti-Human ING4 Polyclonal Antibody | anti-ING4 antibody

Goat Polyclonal to Human ING4

Cat. Num. AAA240554

• Gene Names: ING4; my036; p29ING4
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: ING4; Polyclonal Antibody; Goat Polyclonal to Human ING4; Anti-ING4 Antibody (aa164-175) IHC-plus; Brain my036 protein; Inhibitor of growth protein 4; My036; p29ING4; Human ING4; anti-ING4 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Specificity: aa 164-175 of Human p29 ING4 protein (Inhibitor of growth family, member 4). This sequence only shows homology to isoform 1 for ING4.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 248

• Applicable Applications for anti-ING4 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA (1:3000 - 1:12000), IF, IHC-P (2.5 ug/ml), WB (1:500 - 1:3000); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antib...
• Target Species: Human
• Immunogen Description: Synthetic peptide from human ING4.
• Immunogen Type: Synthetic peptide
• Immunogen: ING4 antibody was raised against synthetic peptide from human ING4.
• Antigen Modification: aa164-175
• Preparation and Storage: +4 degree C or -20 degree C, Avoid repeated freezing and thawing.

Immunohistochemistry (IHC)

(Anti-ING4 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Anti-p29 ING4 Polyclonal Antibody - Western Blot. Anti-p29 ING4 polyclonal antibody detects ING4 protein by Western blot of over expressed cell lysates. This antibody was used at 1.0 ug/ml to detect ING4 expression in control (-) and transformed U2OS and HeLa cell lysates. A predominant band corresponding to p29 ING4 is only seen in lysates from transformed cells. Personnel Communication, Motoko Unoki, NCI, NIH.)

Western Blot (WB)

(Anti-p29 ING4 Polyclonal Antibody - Western Blot. Anti-p29 ING4 polyclonal antibody detects ING4 protein by western blot. This antibody was used at 1.0 ug/ml to detect ING4 (lane 2) present in a U2OS whole cell lysate over expressing the protein. A control lysate (lane 3) shows no background staining. Comparison to MW markers (lane 1) indicates detection of a single band at ~29 kD corresponding to ING4. A 4-20% TRIS-glycine gradient gel was used to separate the protein by SDS-PAGE under reducing conditions. The protein was transferred to nitrocellulose using standard methods. After blocking using 5% non-fat dry milk in PBS, the membrane was probed with the primary antibody overnight at 4° C followed by washes and reaction with a 1:20000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] (code for 45 min at room temperature. LICORs Odyssey Infrared Imaging System was used to scan and process the image. Other detection systems will yield similar results.)

NCBI and Uniprot Product Information

• NCBI GI #: 38201670
• NCBI GeneID: 51147
• NCBI Accession #: NP_057246.2
• NCBI GenBank Nucleotide #: NM_016162.3
• UniProt Accession #: Q9UNL4; Q0EF62; Q0EF63; Q4VBQ6; Q96E15; Q9H3J0; A4KYM4; A4KYM6; D3DUR8
• Molecular Weight: 20,482 Da
• NCBI Official Full Name: inhibitor of growth protein 4 isoform 1
• NCBI Official Synonym Full Names: inhibitor of growth family, member 4
• NCBI Official Symbol: ING4
• NCBI Official Synonym Symbols: my036; p29ING4
• NCBI Protein Information: inhibitor of growth protein 4; brain my036 protein; candidate tumor suppressor p33 ING1 homolog
• UniProt Protein Name: Inhibitor of growth protein 4
• Protein Family: Inhibitor of growth protein
• UniProt Gene Name: ING4
• UniProt Entry Name: ING4_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind TP53 and EP300/p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. Multiple alternatively spliced transcript variants have been observed that encode distinct proteins. [provided by RefSeq, Jul 2008]

Uniprot Description

ING4: similar to ING1, a tumor suppressor protein that can interact with p53, inhibit cell growth, and induce apoptosis. Contains a PHD-finger, which is a common motif in proteins involved in chromatin remodeling. This protein can bind p53 and p300, a component of the histone acetyl transferase complex, suggesting its involvement in the TP53-dependent regulatory pathway. Alternatively spliced transcript variants have been observed, but the biological validity of them has not been determined.

Protein type: Transcription factor; Tumor suppressor

Chromosomal Location of Human Ortholog: 12p13.31

Cellular Component: nucleoplasm; nucleus; histone acetyltransferase complex

Molecular Function: protein binding; zinc ion binding; transcription coactivator activity; methylated histone residue binding

Biological Process: negative regulation of cell proliferation; establishment and/or maintenance of chromatin architecture; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; protein amino acid acetylation; apoptosis; positive regulation of apoptosis; DNA replication; negative regulation of transcription, DNA-dependent; cell cycle arrest; negative regulation of growth