Western Blot (WB)
(Western blot analysis ING2 using HeLa whole cell lysates)
Rabbit anti-Human ING2 Polyclonal Antibody | anti-ING2 antibody
ING2 Antibody
Gene Names
ING2; ING1L; p33ING2
Reactivity
Human
Applications
Western Blot, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ING2; Polyclonal Antibody; ING2 Antibody; ING 1 like tumor suppressor protein; ING 1L; ING 2; ING1 like tumor suppressor protein; ING1L; ING1Lp; ING2_HUMAN; Inhibitor of growth 1 like; Inhibitor of growth 1 like protein; Inhibitor of growth 1-like protein; Inhibitor of growth family member 1 like; Inhibitor of growth family member 2; Inhibitor of growth protein 2; p32; p33 ING2; p33ING2; anti-ING2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
ING2 antibody detects endogenous levels of total ING2
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
240
Applicable Applications for anti-ING2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:1000-3000
Immunogen
A synthesized peptide derived from human ING2
Subcellular Location
Nucleus. Predominantly Nuclear. Localized to chromatin and nuclear matrix. Upon reduced PtdIns(5)P levels seems to be released from chromatin and, at least partially, translocated to the cytoplasm.
Tissue Specificity
Widely expressed. Higher expressed in colon-cancer tumor than in normal colon tissues.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis ING2 using HeLa whole cell lysates)
Western Blot (WB)
(Western blot analysis ING2 using HeLa whole cell lysates)
Related Product Information for anti-ING2 antibody
Function: Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
Subunit Structure: Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of a mSin3A-like complex at least consisting of SIN3A, HDAC1, HDAC2, RBBP4/RbAp48, RBBP7/RbAp46, SAP30 and ING2.
Post-translational Modifications: Sumoylation enhances its association with SIN3A and is required for binding to some target gene promoters, this is the case for TMEM71.
Similarity: The PHD-type zinc finger mediates the binding to H3K4me3.The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns5P). Belongs to the ING family.
Subunit Structure: Interacts with H3K4me3 and to a lesser extent with H3K4me2. Component of a mSin3A-like complex at least consisting of SIN3A, HDAC1, HDAC2, RBBP4/RbAp48, RBBP7/RbAp46, SAP30 and ING2.
Post-translational Modifications: Sumoylation enhances its association with SIN3A and is required for binding to some target gene promoters, this is the case for TMEM71.
Similarity: The PHD-type zinc finger mediates the binding to H3K4me3.The polybasic region (PBR) is responsive to the binding to phosphoinositides (PtdInsPs), including phosphatidylinositol 5-phosphate (PtdIns5P). Belongs to the ING family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 33 kDa
Predicted: 33 kDa
Predicted: 33 kDa
NCBI Official Full Name
inhibitor of growth protein 2 isoform 2
NCBI Official Synonym Full Names
inhibitor of growth family member 2
NCBI Official Symbol
ING2
NCBI Official Synonym Symbols
ING1L; p33ING2
NCBI Protein Information
inhibitor of growth protein 2
UniProt Protein Name
Inhibitor of growth protein 2
Protein Family
UniProt Gene Name
ING2
UniProt Synonym Gene Names
ING1L; ING1Lp
NCBI Description
This gene is a member of the inhibitor of growth (ING) family. Members of the ING family associate with and modulate the activity of histone acetyltransferase (HAT) and histone deacetylase (HDAC) complexes and function in DNA repair and apoptosis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]
Uniprot Description
Seems to be involved in p53/TP53 activation and p53/TP53-dependent apoptotic pathways, probably by enhancing acetylation of p53/TP53. Component of a mSin3A-like corepressor complex, which is probably involved in deacetylation of nucleosomal histones. ING2 activity seems to be modulated by binding to phosphoinositides (PtdInsPs).
Research Articles on ING2
Similar Products
Product Notes
The ING2 ing2 (Catalog #AAA9608647) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ING2 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ING2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:1000-3000. Researchers should empirically determine the suitability of the ING2 ing2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ING2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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