Rabbit Influenza A H1N1 Matrix protein 1/M1 Polyclonal Antibody | anti-M1 antibody

Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 Antibody, Rabbit PAb, Antigen Affinity Purified

Cat. Num. AAA8110559

• Reactivity: H1N1
• Applications: Western Blot, ELISA
• Purity: Protein A & Antigen Affinity
• Synonyms: Influenza A H1N1 Matrix protein 1/M1; Polyclonal Antibody; Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 Antibody; Rabbit PAb; Antigen Affinity Purified; cholinergic receptor; muscarinic 1; anti-M1 antibody

• Host: Rabbit
• Reactivity: H1N1
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-M1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; ELISA: 1:5000-1:10,000; Antibody's applications haven't been validated with corresponding virus positive samples.
• Immunogen: Recombinant Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 Protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1. Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 specific IgG was purified by Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1/M1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1 / M1 rabbit polyclonal antibody at 1:1000 dilution.Sample:Influenza A H1N1 (A/Puerto Rico/8/34/Mount Sinai) Matrix protein 1 / M1 Recombinant ProteinLane A: 30ngSecondaryHRP Conjugated Goat anti-rabbit IgG (H+L) at 1/1000 dilutionPerformed under reducing conditions.)

Related Product Information for anti-M1 antibody

The Influenza virus matrix protein 1 (M1) is a matrix protein of the influenza virus. M1 protein has been shown to play a crucial role in virus replication, assembly, and budding. It forms a coat inside the viral envelope. This is a bifunctional membrane/RNA-binding protein that mediates the encapsidation of RNA-nucleoprotein cores into the membrane envelope. M1 consists of two domains connected by a linker sequence. The N-terminal domain has a multi-helical structure. The C-terminal domain also contains an alpha-helical structure. The M1 protein is the most abundant structural protein in influenza A virus particles. M1 protein of the influenza A virus plays multiple roles in virion assembly and infection. M1 protein was a candidate antigen for a broad-spectrum influenza virus vaccine and the adjuvant chitosan significantly improved the efficacy of the M1 vaccine.

NCBI and Uniprot Product Information

• NCBI GI #: 8486123
• NCBI GeneID: 956527
• NCBI Accession #: NP_040978.1
• NCBI GenBank Nucleotide #: NC_002016.1
• UniProt Accession #: P03485; Q20N36; Q77HF4; A4GXH8
• Molecular Weight: 11,045 Da
• NCBI Official Full Name: matrix protein 1
• NCBI Official Symbol: M1
• NCBI Protein Information: M1
• UniProt Protein Name: Matrix protein 1
• Protein Family: Germanicol synthase
• UniProt Gene Name: M
• UniProt Synonym Gene Names: M1

Uniprot Description

Plays critical roles in virus replication, from virus entry and uncoating to assembly and budding of the virus particle. M1 binding to ribonucleocapsids (RNPs) in nucleus seems to inhibit viral transcription. Interaction of viral NEP with M1-RNP is thought to promote nuclear export of the complex, which is targeted to the virion assembly site at the apical plasma membrane in polarized epithelial cells. Interactions with NA and HA may bring M1, a non-raft-associated protein, into lipid rafts. Forms a continuous shell on the inner side of the lipid bilayer in virion, where it binds the RNP. During virus entry into cell, the M2 ion channel acidifies the internal virion core, inducing M1 dissociation from the RNP. M1-free RNPs are transported to the nucleus, where viral transcription and replication can take place.