Western Blot (WB)
(Anti-IDO1 antibody, MBS175210, Western blottingAll lanes: Anti IDO1 (MBS175210) at 0.5ug/mlLane 1: SMMC Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: 293T Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD )
Rabbit Indoleamine 2, 3-dioxygenase Polyclonal Antibody | anti-IDO1 antibody
Anti-Indoleamine 2, 3-dioxygenase antibody
Concentration: 0.1-0.5ug/ml
Tested Species: Hu
Tested Species: In-house tested species with positive results.
Predicted Species: Species predicted to be fit for the product based on sequence similarities.
Other applications have not been tested.
Optimal dilutions shold be determined by end users.
Western Blot (WB)
(Anti-IDO1 antibody, MBS175210, Western blottingAll lanes: Anti IDO1 (MBS175210) at 0.5ug/mlLane 1: SMMC Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: 293T Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD )
Western Blot (WB)
(Anti-IDO1 antibody, MBS175210, Western blottingAll lanes: Anti IDO1 (MBS175210) at 0.5ug/mlLane 1: SMMC Whole Cell Lysate at 40ugLane 2: A549 Whole Cell Lysate at 40ugLane 3: Human Placenta Tissue Lysate at 50ugLane 4: SW620 Whole Cell Lysate at 40ugLane 5: U87 Whole Cell Lysate at 40ugLane 6: 293T Whole Cell Lysate at 40ugLane 7: A431 Whole Cell Lysate at 40ugLane 8: HELA Whole Cell Lysate at 40ugLane 9: COLO320 Whole Cell Lysate at 40ugPredicted bind size: 45KDObserved bind size: 45KD )
Background: IDO1(INDOLEAMINE 2,3-DIOXYGENASE), INDO or IDO, is an immunomodulatory enzyme produced by some alternatively activated macrophages and other immunoregulatory cells. This enzyme catalyzes the degradation of the essential amino acid L-tryptophan to N-formyl-kynurenine. By fluorescence in situ hybridization, the assignment is narrowed to chromosome 8p12-p11. INDO Interferon-gamma has an antiproliferative effect on many tumor cells and inhibits intracellular pathogens such as Toxoplasma and chlamydia, at least partly because of the induction of indoleamine 2,3-dioxygenase. During inflammation, IDO is upregulated in dendritic cells and phagocytes by proinflammatory stimuli, most notably IFNG, and the enzyme then uses superoxide as a 'cofactor' for oxidative cleavage of the indole ring of tryptophan, yielding an intermediate that deformylates to L-kynurenine.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes indoleamine 2,3-dioxygenase (IDO) - a heme enzyme that catalyzes the first and rate-limiting step in tryptophan catabolism to N-formyl-kynurenine. This enzyme acts on multiple tryptophan substrates including D-tryptophan, L-tryptophan, 5-hydroxy-tryptophan, tryptamine, and serotonin. This enzyme is thought to play a role in a variety of pathophysiological processes such as antimicrobial and antitumor defense, neuropathology, immunoregulation, and antioxidant activity. Through its expression in dendritic cells, monocytes, and macrophages this enzyme modulates T-cell behavior by its peri-cellular catabolization of the essential amino acid tryptophan.[provided by RefSeq, Feb 2011]
Uniprot Description
INDO: Catalyzes the cleavage of the pyrrol ring of tryptophan and incorporates both atoms of a molecule of oxygen. Belongs to the indoleamine 2,3-dioxygenase family.
Protein type: EC 1.13.11.52; Oxidoreductase; Amino Acid Metabolism - tryptophan; Cell cycle regulation
Chromosomal Location of Human Ortholog: 8p12-p11
Cellular Component: smooth muscle contractile fiber; stereocilium bundle; cytosol
Molecular Function: electron carrier activity; metal ion binding; indoleamine 2,3-dioxygenase activity; heme binding; tryptophan 2,3-dioxygenase activity
Biological Process: regulation of activated T cell proliferation; positive regulation of T-helper 2 type immune response; positive regulation of chronic inflammatory response; negative regulation of interleukin-10 production; positive regulation of interleukin-12 production; tryptophan catabolic process to kynurenine; response to lipopolysaccharide; female pregnancy; negative regulation of T cell proliferation; cytokine production during acute inflammatory response; tryptophan catabolic process; multicellular organismal response to stress; positive regulation of T cell tolerance induction
Research Articles on IDO1
Similar Products
Product Notes
The IDO1 ido1 (Catalog #AAA175210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-Indoleamine 2, 3-dioxygenase antibody reacts with Reacts with: Human and may cross-react with other species as described in the data sheet. AAA Biotech's Indoleamine 2, 3-dioxygenase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot: Concentration: 0.1-0.5ug/ml Tested Species: Hu Tested Species: In-house tested species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions shold be determined by end users. Researchers should empirically determine the suitability of the IDO1 ido1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Indoleamine 2, 3-dioxygenase, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
