Rabbit anti-Human, Mouse Indian Hedgehog Polyclonal Antibody | anti-IHH antibody

Indian Hedgehog, CT (IHH, Indian Hedgehog Protein, HHG-2) (MaxLight 550)

Cat. Num. AAA6481392

• Gene Names: Ihh; HHG-2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A/G Affinity Chromatography
• Synonyms: Indian Hedgehog; Polyclonal Antibody; CT (IHH; Indian Hedgehog Protein; HHG-2) (MaxLight 550); anti-IHH antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human Indian Hedgehog Species Crossreactivity: mouse
• Purity/Purification: Purified by Protein A/G Affinity Chromatography
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight550.
• Sequence Length: 411

• Applicable Applications for anti-IHH antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 166-200 in the C-terminal region of human Indian Hedgehog, UniProt Accession #Q14623. Species sequence homology: chiken, zebrafish and Xenopus
• Conjugate: MaxLight550
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight550 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of HL60 cell line lysates (35ug/lane) usingMBS626477. Ihh (arrow) was detected usingI0797-30B.)

Related Product Information for anti-IHH antibody

Ihh (Indian hedgehog) is a critical and possibly direct regulator of joint development. In its absence, distribution and function of Gdf5-expressing interzone-associated cells are abnormal.

Product Categories/Family for anti-IHH antibody

Antibodies; Transcription Factors

References

1. Kobayashi, T., Proc. Natl. Acad. Sci. USA 105(6), 1949-1954 (2008). 2. Gat-Yablonski, G., J. Pediatr. Endocrinol. Metab. 20(11), 1215-1222 (2007). 3. Koyama, E., Ann. N. Y. Acad. Sci. 1116, 100-112 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 922304384
• NCBI GeneID: 16147
• NCBI Accession #: NP_034674
• NCBI GenBank Nucleotide #: NP_034674.2
• UniProt Accession #: Q80XI9; P97812
• Molecular Weight: 49.445
• NCBI Official Full Name: indian hedgehog protein isoform 1 preproprotein
• NCBI Official Synonym Full Names: Indian hedgehog
• NCBI Official Symbol: Ihh
• NCBI Official Synonym Symbols: HHG-2
• NCBI Protein Information: indian hedgehog protein
• UniProt Protein Name: Hedgehog protein
• Protein Family: Indian hedgehog protein
• UniProt Gene Name: Ihh
• UniProt Entry Name: Q80XI9_MOUSE

Uniprot Description

IHH: Intercellular signal essential for a variety of patterning events during development. Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. Implicated in endochondral ossification: may regulate the balance between growth and ossification of the developing bones. Induces the expression of parathyroid hormone-related protein (PTHRP). Expressed in embryonic lung, and in adult kidney and liver. Belongs to the hedgehog family.

Cellular Component: proteinaceous extracellular matrix; membrane; plasma membrane; extracellular region

Molecular Function: peptidase activity; protein binding; hydrolase activity; metal ion binding; patched binding; calcium ion binding

Biological Process: vitelline membrane formation; proteoglycan metabolic process; multicellular organismal development; positive regulation of collagen biosynthetic process; cell maturation; cell fate specification; proteolysis; embryonic pattern specification; positive regulation of T cell differentiation in the thymus; smooth muscle development; cell-cell signaling; vasculature development; embryonic digestive tract morphogenesis; regulation of cell differentiation; regulation of growth; pancreas development; positive regulation of cell proliferation; positive regulation of mesenchymal cell proliferation; positive regulation of smoothened signaling pathway; cell communication; heart looping; cell differentiation; skeletal development; bone resorption; negative regulation of immature T cell proliferation in the thymus; smoothened signaling pathway; ossification; camera-type eye development; intein-mediated protein splicing; in utero embryonic development; multicellular organism growth; negative regulation of eye pigmentation; morphogenesis of a branching structure; negative regulation of T cell differentiation in the thymus; pattern specification process; negative regulation of signal transduction; patterning of blood vessels; osteoblast differentiation; negative regulation of cell differentiation; embryonic development; positive regulation of chondrocyte differentiation; cartilage development; neuron development; positive regulation of transcription from RNA polymerase II promoter; embryonic camera-type eye morphogenesis; embryonic digit morphogenesis; positive regulation of alpha-beta T cell differentiation; positive regulation of epithelial cell proliferation; negative regulation of apoptosis; negative regulation of alpha-beta T cell differentiation