Western Blot (WB)
(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS625147.This demonstrates that MBS625147 detected the Ihh protein (arrow).)
Rabbit anti-Human, Mouse Ihh Polyclonal Antibody | anti-Ihh antibody
Ihh, NT (IHH, Indian Hedgehog Protein, HHG-2) (AP)
Gene Names
Ihh; HHG-2
Reactivity
Human, Mouse
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
Purified by Protein A and Peptide Affinity Chromatography.
Synonyms
Ihh; Polyclonal Antibody; NT (IHH; Indian Hedgehog Protein; HHG-2) (AP); anti-Ihh antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IHH. Species Crossreactivity: mouse
Purity/Purification
Purified by Protein A and Peptide Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Sequence Length
411
Applicable Applications for anti-Ihh antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 1-30 in the N-terminal region of human IHH. UniProt Accession: Q14623
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS625147.This demonstrates that MBS625147 detected the Ihh protein (arrow).)
Western Blot (WB)
(Western Blot analysis in mouse liver tissue lysates (35ug/lane) usingMBS625147.This demonstrates that MBS625147 detected the Ihh protein (arrow).)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted withMBS625147, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS625147 for immunohistochemistry.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted withMBS625147, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of MBS625147 for immunohistochemistry.)
Related Product Information for anti-Ihh antibody
Ihh (Indian hedgehog) is a critical and possibly direct regulator of joint development. In its absence, distribution and function of Gdf5-expressing interzone-associated cells are abnormal.
Product Categories/Family for anti-Ihh antibody
References
1. Kobayashi, T., Proc. Natl. Acad. Sci. USA 105(6), 1949-1954 (2008). 2. Gat-Yablonski, G., J. Pediatr. Endocrinol. Metab. 20(11), 1215-1222 (2007). 3. Koyama, E., Ann. N. Y. Acad. Sci. 1116, 100-112 (2007).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49.445
NCBI Official Full Name
indian hedgehog protein isoform 1 preproprotein
NCBI Official Synonym Full Names
Indian hedgehog
NCBI Official Symbol
Ihh
NCBI Official Synonym Symbols
HHG-2
NCBI Protein Information
indian hedgehog protein
UniProt Protein Name
Indian hedgehog protein
Protein Family
UniProt Gene Name
IHH
UniProt Synonym Gene Names
IHH
UniProt Entry Name
IHH_HUMAN
Research Articles on Ihh
Similar Products
Product Notes
The Ihh ihh (Catalog #AAA6481382) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Ihh, NT (IHH, Indian Hedgehog Protein, HHG-2) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Ihh can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the Ihh ihh for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ihh, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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