Atrial Natriuretic Peptide (ANP) Recombinant Protein | ANP recombinant protein

Recombinant Atrial Natriuretic Peptide (ANP)

Cat. Num. AAA2012467

• Gene Names: Nppa; ANF; ANP; Pnd; RATANF
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Atrial Natriuretic Peptide (ANP); Recombinant Atrial Natriuretic Peptide (ANP); ANP recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-NPVYSA VSNTDLMDFK NLLDHLEEKM PVEDEVMPPQ ALSEQTDEAG AALSSLSEVP PWTGEVNPSQ RDGGALGRGP WDPSDRSALL KSKLRALLAG PRSLRRSSCF GGRIDRIGAQ SGLGCNSFRY RR
• Sequence Length: 152

• Applicable Applications for ANP recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Asn25~Arg152 (Accession # P01161) with N-terminal His-Tag
• Subcellular Location: Secreted
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.4
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6981278
• NCBI GeneID: 24602
• NCBI Accession #: NP_036744.1
• NCBI GenBank Nucleotide #: NM_012612.2
• UniProt Accession #: P01161
• Molecular Weight: 15.4kDa
• NCBI Official Full Name: natriuretic peptides A
• NCBI Official Synonym Full Names: natriuretic peptide A
• NCBI Official Symbol: Nppa
• NCBI Official Synonym Symbols: ANF; ANP; Pnd; RATANF
• NCBI Protein Information: natriuretic peptides A; prepronatriodilatin; atrial natriuretic factor; atrial natriuretic peptide; natriuretic peptide precursor type A; Natriuretic peptide precursor A (pronatriodilatin, also Anf, Pnd)
• UniProt Protein Name: Natriuretic peptides A
• Protein Family: Andropin
• UniProt Gene Name: Nppa
• UniProt Synonym Gene Names: ANF; ANP
• UniProt Entry Name: ANF_RAT

NCBI Description

peptide involved in the control of fluid volume and vascular function [RGD, Feb 2006]

Uniprot Description

NPPA: Hormone playing a key role in cardiovascular homeostasis through regulation of natriuresis, diuresis, and vasodilation. Also plays a role in female pregnancy by promoting trophoblast invasion and spiral artery remodeling in uterus. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Defects in NPPA are the cause of familial atrial fibrillation type 6 (ATFB6). Atrial fibrillation is a common disorder of cardiac rhythm that is hereditary in a small subgroup of patients. It is characterized by disorganized atrial electrical activity, progressive deterioration of atrial electromechanical function and ineffective pumping of blood into the ventricles. It can be associated with palpitations, syncope, thromboembolic stroke, and congestive heart failure. Belongs to the natriuretic peptide family.

Protein type: Secreted, signal peptide; Hormone; Secreted