Human Integrin alpha-9 ELISA Kit | ITGA9 elisa kit

Human Integrin alpha-9 ELISA Kit

Cat. Num. AAA764614

• Gene Names: ITGA9; RLC; ITGA4L; ALPHA-RLC
• Reactivity: Human
• Synonyms: Integrin alpha-9; Human Integrin alpha-9 ELISA Kit; ITGA9/ITGA4L/RLC-alpha/ALPHA-RLC/integrin alpha-9/Integrin alpha-RLC/integrin; alpha 9/ITGA4L/RLC; ITGA9 elisa kit

• Reactivity: Human
• Sequence Length: 1035

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ITGA9 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human ITGA9 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ITGA9 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ITGA9 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human ITGA9 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 52485941
• NCBI GeneID: 3680
• NCBI Accession #: NP_002198.2
• NCBI GenBank Nucleotide #: NM_002207.2
• Molecular Weight: 114,489 Da
• NCBI Official Full Name: integrin alpha-9
• NCBI Official Synonym Full Names: integrin subunit alpha 9
• NCBI Official Symbol: ITGA9
• NCBI Official Synonym Symbols: RLC; ITGA4L; ALPHA-RLC
• NCBI Protein Information: integrin alpha-9
• UniProt Protein Name: Integrin alpha-9
• Protein Family: Integrin
• UniProt Gene Name: ITGA9
• UniProt Entry Name: ITA9_HUMAN

NCBI Description

This gene encodes an alpha integrin. Integrins are heterodimeric integral membrane glycoproteins composed of an alpha chain and a beta chain that mediate cell-cell and cell-matrix adhesion. The protein encoded by this gene, when bound to the beta 1 chain, forms an integrin that is a receptor for VCAM1, cytotactin and osteopontin. Expression of this gene has been found to be upregulated in small cell lung cancers. [provided by RefSeq, Jul 2008]

Uniprot Description

ITGA9: Integrin alpha-9/beta-1 is a receptor for VCAM1, cytotactin and osteopontin. It recognizes the sequence A-E-I-D-G- I-E-L in cytotactin. Belongs to the integrin alpha chain family.

Protein type: Cell adhesion; Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: basal plasma membrane; integrin complex; plasma membrane

Molecular Function: collagen binding; laminin binding; metal ion binding

Biological Process: axon guidance; cell adhesion; extracellular matrix organization and biogenesis; integrin-mediated signaling pathway; neutrophil chemotaxis; wound healing