Rabbit anti-Human IMPDH1 Polyclonal Antibody | anti-IMPDH1 antibody

IMPDH1, NT (IMPDH1, IMPD1, Inosine-5'-monophosphate dehydrogenase 1, IMPDH-I) (Azide free) (HRP)

Cat. Num. AAA6310447

• Gene Names: IMPDH1; IMPD; RP10; IMPD1; LCA11; IMPDH-I; sWSS2608
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: IMPDH1; Polyclonal Antibody; NT (IMPDH1; IMPD1; Inosine-5'-monophosphate dehydrogenase 1; IMPDH-I) (Azide free) (HRP); IMPDH-I; anti-IMPDH1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 514

• Applicable Applications for anti-IMPDH1 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: IMPDH1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 83-113 amino acids from the N-terminal region of human IMPDH1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(IMPDH1 Antibody (N-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the IMPDH1 antibody detected the IMPDH1 protein (arrow).)

Immunohistochemistry (IHC)

(IMPDH1 Antibody (N-term) (AP14691a)immunohistochemistry analysis in formalin fixed and paraffin embedded human breast tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IMPDH1 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-IMPDH1 antibody

The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene.

Product Categories/Family for anti-IMPDH1 antibody

Antibodies; Enzymes; Dehydrogenase

References

Ohmann, E.L., et al. Pediatr Transplant 14(7):891-895(2010). Gensburger, O., et al. Pharmacogenet. Genomics 20(9):537-543(2010). Kagaya, H., et al. Basic Clin. Pharmacol. Toxicol. 107(2):631-636(2010). Ohmann, E.L., et al. J. Heart Lung Transplant. 29(5):509-516(2010). Shumei, L., et al. Adv. Exp. Med. Biol. 664, 293-297 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 217035146
• NCBI GeneID: 3614
• NCBI Accession #: NP_001136045.1; NP_899066.1; NP_001096075.1
• NCBI GenBank Nucleotide #: NP_001136045.1
• UniProt Accession #: P20839
• NCBI Official Full Name: inosine-5'-monophosphate dehydrogenase 1 isoform e
• NCBI Official Synonym Full Names: inosine monophosphate dehydrogenase 1
• NCBI Official Symbol: IMPDH1
• NCBI Official Synonym Symbols: IMPD; RP10; IMPD1; LCA11; IMPDH-I; sWSS2608
• NCBI Protein Information: inosine-5'-monophosphate dehydrogenase 1
• UniProt Protein Name: Inosine-5'-monophosphate dehydrogenase 1
• Protein Family: Inosine-5'-monophosphate dehydrogenase
• UniProt Gene Name: IMPDH1
• UniProt Entry Name: IMDH1_HUMAN

NCBI Description

The protein encoded by this gene acts as a homotetramer to regulate cell growth. The encoded protein is an enzyme that catalyzes the synthesis of xanthine monophosphate (XMP) from inosine-5'-monophosphate (IMP). This is the rate-limiting step in the de novo synthesis of guanine nucleotides. Defects in this gene are a cause of retinitis pigmentosa type 10 (RP10). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

IMPDH1: Catalyzes the conversion of inosine 5'-phosphate (IMP) to xanthosine 5'-phosphate (XMP), the first committed and rate- limiting step in the de novo synthesis of guanine nucleotides, and therefore plays an important role in the regulation of cell growth. Could also have a single-stranded nucleic acid-binding activity and could play a role in RNA and/or DNA metabolism. It may also have a role in the development of malignancy and the growth progression of some tumors. Defects in IMPDH1 are the cause of retinitis pigmentosa type 10 (RP10). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP10 inheritance is autosomal dominant. Defects in IMPDH1 are the cause of Leber congenital amaurosis type 11 (LCA11). LCA11 is a severe dystrophy of the retina, typically becoming evident in the first years of life. Visual function is usually poor and often accompanied by nystagmus, sluggish or near-absent pupillary responses, photophobia, high hyperopia and keratoconus. Belongs to the IMPDH/GMPR family. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Xenobiotic Metabolism - drug metabolism - other enzymes; EC 1.1.1.205; Nucleotide Metabolism - purine; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q31.3-q32

Cellular Component: nucleoplasm; cytoplasm; cell junction; nucleus; cytosol

Molecular Function: DNA binding; nucleic acid binding; RNA binding; metal ion binding; nucleotide binding; IMP dehydrogenase activity

Biological Process: lymphocyte proliferation; purine ribonucleoside monophosphate biosynthetic process; nucleobase, nucleoside and nucleotide metabolic process; GMP biosynthetic process; purine base metabolic process

Disease: Leber Congenital Amaurosis 11; Retinitis Pigmentosa 10