Western Blot (WB)
(Western blot analysis of IMMT Antibody (Center) in Hela, NCI-H460, CEM cell line lysates (35ug/lane). IMMT (arrow) was detected using the purified Pab.)
Rabbit anti-Human IMMT Polyclonal Antibody | anti-IMMT antibody
IMMT, ID (Mitochondrial Inner Membrane Protein, Mitofilin, p87/89, Cell Proliferation-inducing Gene 4 Protein, HMP, PIG4, PIG52)
Reactivity
Human
Applications
ELISA, Western Blot
Purity
PurifiedPurified by ammonium sulfate precipitation.
Synonyms
IMMT; Polyclonal Antibody; ID (Mitochondrial Inner Membrane Protein; Mitofilin; p87/89; Cell Proliferation-inducing Gene 4 Protein; HMP; PIG4; PIG52); Anti -IMMT; anti-IMMT antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human IMMT.
Purity/Purification
Purified
Purified by ammonium sulfate precipitation.
Purified by ammonium sulfate precipitation.
Form/Format
Supplied as a liquid in PBS, 0.09% sodium azide.
Applicable Applications for anti-IMMT antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100
Immunogen
Synthetic peptide selected from the Center region of human IMMT (KLH).
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Western Blot (WB)
(Western blot analysis of IMMT Antibody (Center) in Hela, NCI-H460, CEM cell line lysates (35ug/lane). IMMT (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of IMMT Antibody (Center) in Hela, NCI-H460, CEM cell line lysates (35ug/lane). IMMT (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with IMMT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human brain tissue reacted with IMMT Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(IMMT Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(IMMT Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Related Product Information for anti-IMMT antibody
Mitochondria are the center of cellular energy production and essential metabolic reactions. As double membrane-bound organelles, mitochondria from different species, tissues, and metabolic states are highly polymorphic in nature, yet exhibit common structural features. The ultrastructural variations in mitochondrial architecture occur mainly due to the differences in the amount and shape of cristae. Abundant cristae are found in mitochondria from tissues where energy demand is high. Analysis of the human heart mitochondrial proteome shows that mitofilin is one of the most abundant mitochondrial proteins. It appears to play an important role in the maintenance of cristae morphology.
Product Categories/Family for anti-IMMT antibody
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
83,678 Da
NCBI Official Full Name
IMMT
UniProt Protein Name
Mitochondrial inner membrane protein
UniProt Gene Name
IMMT
UniProt Synonym Gene Names
HMP
UniProt Entry Name
IMMT_HUMAN
Uniprot Description
IMMT: 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Membrane protein, integral
Chromosomal Location of Human Ortholog: 2p11.2|2
Cellular Component: mitochondrion; membrane; mitochondrial inner membrane; integral to membrane
Molecular Function: protein binding
Biological Process: mitochondrial calcium ion homeostasis; response to cold
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Product Notes
The IMMT immt (Catalog #AAA626363) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IMMT, ID (Mitochondrial Inner Membrane Protein, Mitofilin, p87/89, Cell Proliferation-inducing Gene 4 Protein, HMP, PIG4, PIG52) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's IMMT can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: ELISA: 1:1,000 Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the IMMT immt for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IMMT, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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