Rabbit ILVA Polyclonal Antibody | anti-ILVA antibody

Rabbit anti-Escherichia coli (strain K12) ILVA Polyclonal Antibody

• Gene Names: ilvA; ECK3764; JW3745
• Reactivity: E Coli (strain K12)
• Applications: ELISA, Western Blot
• Purity: Protein A/G
• Synonyms: ILVA; Polyclonal Antibody; Rabbit anti-Escherichia coli (strain K12) ILVA Polyclonal Antibody; ILVA Antibody; L-threonine dehydratase biosynthetic IlvA; EC 4.3.1.19; Threonine deaminase; ilvA b3772 JW3745; anti-ILVA antibody

• Host: Rabbit
• Reactivity: E Coli (strain K12)
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Protein A/G
• Form/Format: Liquid; Preservative: 0.03% Proclin 300; Constituents: 50% Glycerol, 0.01M PBS, pH7.4

• Applicable Applications for anti-ILVA antibody: ELISA (EIA), WB (Western Blot) (ensure identification of antigen)
• Immunogen: Recombinant Escherichia coli (strain K12) ilvA protein
• Conjugation: Non-conjugate
• Modification: Unmodified
• Deliverables: 1. 200ug recombinant immunogen protein/peptide (positive control).; 2. 1mL preimmune serum (negative control); 3. 10mg rabbit polyclonal antibody purified by Protein A/G.
• Quality Guarantee: 1. Antibody purity >90% confirmed by SDS-PAGE.; 2. Antibody titer > 1: 64,000 confirmed by ELISA; 3. Western Blot positive for the immunogen protein (Not applicable for synthetic peptide).
• Complementary Antibody: Customer can choose 1 FREE antibody from the list below. Please specify the catalog # when order:; * 6*His Monoclonal Antibody #MBS1491670; * DYKDDDDK tag Monoclonal Antibody #MBS1493946; * GAPDH Monoclonal Antibody #MBS1495524; * GFP Monoclonal Antibody #MBS1496050; * Myc tag Monoclonal Antibody #MBS1497694; * GST Monoclonal Antibody #MBS1497871; * Sumo tag Monoclonal Antibody #MBS1498878; * Goat Anti-Rabbit IgG (H+L) Antibody; HRP conjugated #MBS7053510; * HA-Tag Monoclonal Antibody #MBS7135631; * E-Tag Monoclonal Antibody #MBS7135632; * TUBA1A Monoclonal Antibody #MBS7135645; * TUBB Monoclonal Antibody #MBS9018332
• Preparation and Storage: Upon receipt, store at -20 degree C or -80 degree C. Avoid repeated freeze.

NCBI and Uniprot Product Information

• NCBI GI #: 16131630
• NCBI GeneID: 948287
• NCBI Accession #: NP_418220.1
• NCBI GenBank Nucleotide #: NC_000913.3
• UniProt Accession #: P04968; Q2M881
• Molecular Weight: 56,195 Da
• NCBI Official Full Name: l-threonine dehydratase, biosynthetic; also known as threonine deaminase
• NCBI Official Symbol: ilvA
• NCBI Official Synonym Symbols: ECK3764; JW3745
• NCBI Protein Information: l-threonine dehydratase, biosynthetic; also known as threonine deaminase
• UniProt Protein Name: L-threonine dehydratase biosynthetic IlvA
• Protein Family: L-threonine dehydratase biosynthetic
• UniProt Gene Name: ilvA

NCBI Description

N-terminal sequence of cloned IlvA shows fMet is clipped, but natural RBS was not used (Eisenstein, 1991) so it is not Verified, but the ATG start is reliable based on comparisons to Salmonella. [More information is available at EcoGene: EG10493]. Threonine deaminase (IlvA) carries out the first step in the synthesis of isoleucine. [More information is available at EcoCyc: EG10493].

Uniprot Description

Catalyzes the anaerobic formation of alpha-ketobutyrate and ammonia from threonine in a two-step reaction. The first step involved a dehydration of threonine and a production of enamine intermediates (aminocrotonate), which tautomerizes to its imine form (iminobutyrate). Both intermediates are unstable and short-lived. The second step is the nonenzymatic hydrolysis of the enamine/imine intermediates to form 2-ketobutyrate and free ammonia. In the low water environment of the cell, the second step is accelerated by RidA.