Rabbit IL3RA Polyclonal Antibody | anti-IL3RA antibody

IL3RA Polyclonal Antibody

Cat. Num. AAA3006931

• Gene Names: IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: IL3RA; Polyclonal Antibody; IL3RA Polyclonal Antibody; Interleukin-3 receptor subunit alpha; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA; CD123; anti-IL3RA antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: IL3RA polyclonal antibody detects endogenous levels of IL3RA protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 378

• Applicable Applications for anti-IL3RA antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to residues in Human IL3RA
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of IL3RA pAb at 1:500 dilutionLane1:The Oviduct tissue lysate of Rat (40ug) Lane2:The Kidney tissue lysate of Mouse (40ug) Lane3:SGC7901 whole cell lysate (40ug) Lane4:LOVO whole cell lysate (40ug))

Western Blot (WB)

(Western Blot (WB) analysis of IL3RA pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:Hela whole cell lysate (40ug) Lane3:PC12 whole cell lysate (40ug) Lane4:MEF whole cell lysate (40ug))

Related Product Information for anti-IL3RA antibody

Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific alpha subunit (IL-3Ralpha) and a signal transducing beta subunit (IL-3Rbeta) common to the IL-3/IL-5/GM-CSF receptors. The alpha subunits are low-affinity ligand-binding proteins while the beta subunits do not themselves bind ligand, but are required for high affinity binding by the alpha subunits. The mouse IL-3 receptor has two distinct beta subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine beta subunits are 91% homologous at the amino acid level but only 56% homologous to the human beta subunit. The carboxy-terminus of the beta subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b.

NCBI and Uniprot Product Information

• NCBI GI #: 417184
• NCBI GeneID: 3563
• UniProt Accession #: P26951
• Molecular Weight: ~ 44kDa
• NCBI Official Full Name: Interleukin-3 receptor subunit alpha
• NCBI Official Synonym Full Names: interleukin 3 receptor subunit alpha
• NCBI Official Symbol: IL3RA
• NCBI Official Synonym Symbols: IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra
• NCBI Protein Information: interleukin-3 receptor subunit alpha
• UniProt Protein Name: Interleukin-3 receptor subunit alpha
• Protein Family: Interleukin-3 receptor
• UniProt Gene Name: IL3RA
• UniProt Synonym Gene Names: IL3R; IL-3 receptor subunit alpha; IL-3R subunit alpha; IL-3R-alpha; IL-3RA
• UniProt Entry Name: IL3RA_HUMAN

NCBI Description

The protein encoded by this gene is an interleukin 3 specific subunit of a heterodimeric cytokine receptor. The receptor is comprised of a ligand specific alpha subunit and a signal transducing beta subunit shared by the receptors for interleukin 3 (IL3), colony stimulating factor 2 (CSF2/GM-CSF), and interleukin 5 (IL5). The binding of this protein to IL3 depends on the beta subunit. The beta subunit is activated by the ligand binding, and is required for the biological activities of IL3. This gene and the gene encoding the colony stimulating factor 2 receptor alpha chain (CSF2RA) form a cytokine receptor gene cluster in a X-Y pseudoautosomal region on chromosomes X or Y. Alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jun 2012]

Uniprot Description

IL3RA: This is a receptor for interleukin-3. Belongs to the type I cytokine receptor family. Type 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: Xp22.3 or Yp11.3

Cellular Component: plasma membrane; integral to membrane

Molecular Function: interleukin-3 receptor activity