Western Blot (WB)
(Figure 1. Western blot analysis of IL1RA/Il1rn using anti-IL1RA/Il1rn antibody (MBS1753356).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat spleen tissue lysatesLane 2: rat brain tissue lysatesLane 3: rat thymus tissue lysatesLane 4: rat kidney tissue lysatesLane 5: rat PC-12 whole cell lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse kidney tissue lysatesLane 8: mouse RAW264. 7 whole cell lysatesLane 9: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-IL1RA/Il1rn antigen affinity purified polyclonal antibody (Catalog # MBS1753356) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for IL1RA/Il1rn at approximately 18KD. The expected band size for IL1RA/Il1rn is at 18KD. )
Rabbit anti-Mouse, Rat IL1RA/Il1rn Polyclonal Antibody | anti-Il1rn antibody
Anti-IL1RA/Il1rn Antibody
IHC-P: 2-5ug/ml|Mouse, Rat|
FC/FACS/FCM: 1-3ug/1x106 cells|Mouse, Rat|
Direct ELISA: 0.1-0.5ug/ml|Mouse|
Western Blot (WB)
(Figure 1. Western blot analysis of IL1RA/Il1rn using anti-IL1RA/Il1rn antibody (MBS1753356).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat spleen tissue lysatesLane 2: rat brain tissue lysatesLane 3: rat thymus tissue lysatesLane 4: rat kidney tissue lysatesLane 5: rat PC-12 whole cell lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse kidney tissue lysatesLane 8: mouse RAW264. 7 whole cell lysatesLane 9: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-IL1RA/Il1rn antigen affinity purified polyclonal antibody (Catalog # MBS1753356) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for IL1RA/Il1rn at approximately 18KD. The expected band size for IL1RA/Il1rn is at 18KD. )
Western Blot (WB)
(Figure 1. Western blot analysis of IL1RA/Il1rn using anti-IL1RA/Il1rn antibody (MBS1753356).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: rat spleen tissue lysatesLane 2: rat brain tissue lysatesLane 3: rat thymus tissue lysatesLane 4: rat kidney tissue lysatesLane 5: rat PC-12 whole cell lysatesLane 6: mouse spleen tissue lysatesLane 7: mouse kidney tissue lysatesLane 8: mouse RAW264. 7 whole cell lysatesLane 9: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-IL1RA/Il1rn antigen affinity purified polyclonal antibody (Catalog # MBS1753356) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # MBS176460) with Tanon 5200 system. A specific band was detected for IL1RA/Il1rn at approximately 18KD. The expected band size for IL1RA/Il1rn is at 18KD. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-IL1RA/Il1rn antibody (MBS1753356).Overlay histogram showing HEPA1-6 cells stained with MBS1753356 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (MBS1753356, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 2. Flow Cytometry analysis of HEPA1-6 cells using anti-IL1RA/Il1rn antibody (MBS1753356).Overlay histogram showing HEPA1-6 cells stained with MBS1753356 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (MBS1753356, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of NRK cells using anti-IL1RA/Il1rn antibody (MBS1753356).Overlay histogram showing NRK cells stained with MBS1753356 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (MBS1753356, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
Flow Cytometry (FC/FACS)
(Figure 3. Flow Cytometry analysis of NRK cells using anti-IL1RA/Il1rn antibody (MBS1753356).Overlay histogram showing NRK cells stained with MBS1753356 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-IL1RA/Il1rn Antibody (MBS1753356, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control. )
2. Patterson D, Jones C, Hart I, Bleskan J, Berger R, Geyer D, Eisenberg SP, Smith MF, Arend WP (January 1993). "The human interleukin-1 receptor antagonist (IL1RN) gene is located in the chromosome 2q14 region". Genomics. 15 (1): 173-6.
3. Steinkasserer A, Spurr NK, Cox S, Jeggo P, Sim RB (July 1992). "The human IL-1 receptor antagonist gene (IL1RN) maps to chromosome 2q14-q21, in the region of the IL-1 alpha and IL-1 beta loci". Genomics. 13(3): 654-7.
NCBI and Uniprot Product Information
Uniprot Description
IL1RN: Inhibits the activity of interleukin-1 by binding to receptor IL1R1 and preventing its association with the coreceptor IL1RAP for signaling. Has no interleukin-1 like activity. Binds functional interleukin-1 receptor IL1R1 with greater affinity than decoy receptor IL1R2; however, the physiological relevance of the latter association is unsure. The intracellular form of IL1RN is predominantly expressed in epithelial cells. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Cytokine; Secreted; Secreted, signal peptide; Vesicle
Cellular Component: extracellular space; cell; cytoplasm; extracellular region; vesicle
Molecular Function: interleukin-1 Type I receptor antagonist activity; interleukin-1 receptor binding; interleukin-1 Type II receptor antagonist activity; interleukin-1, Type II receptor binding; cytokine activity; interleukin-1, Type I receptor binding; interleukin-1 receptor antagonist activity
Biological Process: positive regulation of JNK activity; negative regulation of glutamate secretion; negative regulation of heterotypic cell-cell adhesion; cytokine and chemokine mediated signaling pathway; response to glucocorticoid stimulus; fever; sensory perception of pain; memory; insulin secretion; acute-phase response; negative regulation of cytokine and chemokine mediated signaling pathway; lipid metabolic process; negative regulation of membrane potential; negative regulation of cell migration; negative regulation of apoptosis
Research Articles on Il1rn
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Product Notes
The Il1rn il1rn (Catalog #AAA1753356) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-IL1RA/Il1rn Antibody reacts with Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's IL1RA/Il1rn can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS/FCM), Direct ELISA (EIA). WB: 0.25-0.5ug/ml|Mouse, Rat| IHC-P: 2-5ug/ml|Mouse, Rat| FC/FACS/FCM: 1-3ug/1x106 cells|Mouse, Rat| Direct ELISA: 0.1-0.5ug/ml|Mouse|. Researchers should empirically determine the suitability of the Il1rn il1rn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL1RA/Il1rn, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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