Rabbit anti-Mouse Il12b Polyclonal Antibody | anti-Il12b antibody

Il12b, ID (Il12b, Interleukin-12 subunit beta, Cytotoxic lymphocyte maturation factor 40kD subunit, IL-12 subunit p40) (Biotin)

Cat. Num. AAA6309862

• Gene Names: Il12b; p40; Il-12b; Il12p40; Il-12p40
• Reactivity: Mouse
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: Il12b; Polyclonal Antibody; ID (Il12b; Interleukin-12 subunit beta; Cytotoxic lymphocyte maturation factor 40kD subunit; IL-12 subunit p40) (Biotin); IL-12 subunit p40; anti-Il12b antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes mouse Il12b.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-Il12b antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 196-222 in the central region of mouse Il12b.
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in mouse heart tissue lysates (35ug/lane) usingMBS6010424. This demonstrates that MBS6010424 detected the Il12b protein (arrow).)

Related Product Information for anti-Il12b antibody

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC (By similarity). Associates with IL23A to form the IL-23 interleukin, an heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to an heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of proinflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.

Product Categories/Family for anti-Il12b antibody

Antibodies; Interleukin

References

1. Kagami, S., et al. J. Immunol. 185(9):5453-5462(2010). 2. Wozniak, T.M., et al. Infect. Immun. 78(10):4187-4194(2010). 3. Lindsay, R.W., et al. J. Immunol. 185(3):1513-1521(2010). 4. Marshall, H.D., et al. J. Immunol. 185(3):1419-1428(2010). 5. Park, Y., et al. J. Immunol. 185(3):1476-1484(2010).

NCBI and Uniprot Product Information

• NCBI GI #: 6680397
• NCBI GeneID: 16160
• NCBI Accession #: NP_032378.1
• NCBI GenBank Nucleotide #: NM_008352.2
• UniProt Accession #: P43432; Q9QUM1
• Molecular Weight: 38,235 Da
• NCBI Official Synonym Full Names: interleukin 12b
• NCBI Official Symbol: Il12b
• NCBI Official Synonym Symbols: p40; Il-12b; Il12p40; Il-12p40
• NCBI Protein Information: interleukin-12 subunit beta
• UniProt Protein Name: Interleukin-12 subunit beta
• Protein Family: Interleukin
• UniProt Gene Name: Il12b
• UniProt Synonym Gene Names: IL-12B; CLMF p40

NCBI Description

This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23. [provided by RefSeq, Dec 2014]

Uniprot Description

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.