Rabbit IL-4 Polyclonal Antibody | anti-Il4 antibody

RABBIT ANTI MOUSE INTERLEUKIN-4

Cat. Num. AAA221266

• Gene Names: Il4; Il-4; BSF-1
• Applications: ELISA, Functional Assay, Western Blot
• Synonyms: IL-4; Polyclonal Antibody; RABBIT ANTI MOUSE INTERLEUKIN-4; anti-Il4 antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0 mg/ml after reconstitution. (varies by lot)
• Sequence Length: 140

• Applicable Applications for anti-Il4 antibody: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Maximum Dilution: 0.5ug/ml;; Functional Assays: Minimum Dilution: 0.25ug/ml; Maximum Dilution: 0.40ug/ml;; Western Blotting: Minimum Dilution: 0.1ug/ml; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
• Immunogen: Recombinant mouse IL-4; Buffer Solution
• Antiserum Preparation: Antisera to mouse IL-4 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG was prepared by affinity chromatography.
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Related Product Information for anti-Il4 antibody

Rabbit anti Mouse Interleukin-4 antibody recognizes mouse interleukin-4 (IL-4), also known as B-cell stimulatory factor 1, a 120 amino acid pleiotropic cytokine cleaved from a 140 amino acid precursor which interacts with multiple cell lineages, including T cells, fibroblasts and macrophages (Crawford et al. 1987). Interleukin-4 is one of the most important factors involved in B cell IgG1 and IgE class switching (Winter et al. 1987). IL-4 acts as a regulator of low affinity Fc receptor for IgE (CD23) expression on both lymphocytes and monocytes (Miyajima et al. 1991, Hudak et al. 1987). IL-4 acts through binding to it's cognate receptor CD124, a receptor which also binds the related cytokine IL-13. Interleukin -4 plays an important role in the alternative activation of murine macrophages as indicated by the ability to potently up-regulate the expression and activity of CD206, the macrophage mannose receptor (Stein et al. 1992).

NCBI and Uniprot Product Information

• NCBI GI #: 10946584
• NCBI GeneID: 16189
• NCBI Accession #: NP_067258.1
• NCBI GenBank Nucleotide #: NM_021283.2
• UniProt Accession #: P07750
• Molecular Weight: 15,834 Da
• NCBI Official Full Name: interleukin-4
• NCBI Official Synonym Full Names: interleukin 4
• NCBI Official Symbol: Il4
• NCBI Official Synonym Symbols: Il-4; BSF-1
• NCBI Protein Information: interleukin-4; B-cell IgG differentiation factor; B-cell growth factor 1; B-cell stimulatory factor 1; IGG1 induction factor; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• Protein Family: Interleukin
• UniProt Gene Name: Il4
• UniProt Synonym Gene Names: Il-4; IL-4; BSF-1
• UniProt Entry Name: IL4_MOUSE

Uniprot Description

IL4: Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Genetic variations in IL4 may be a cause of susceptibility to ischemic stroke (ISCHSTR); also known as cerebrovascular accident or cerebral infarction. A stroke is an acute neurologic event leading to death of neural tissue of the brain and resulting in loss of motor, sensory and/or cognitive function. Ischemic strokes, resulting from vascular occlusion, is considered to be a highly complex disease consisting of a group of heterogeneous disorders with multiple genetic and environmental risk factors. Belongs to the IL-4/IL-13 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide; Cytokine; Motility/polarity/chemotaxis; Cell cycle regulation

Cellular Component: extracellular space; extracellular region; external side of plasma membrane

Molecular Function: growth factor activity; hematopoietin/interferon-class (D200-domain) cytokine receptor binding; cytokine activity; interleukin-4 receptor binding

Biological Process: positive regulation of isotype switching to IgG isotypes; negative regulation of osteoclast differentiation; positive regulation of transcription, DNA-dependent; regulation of proton transport; microglial cell activation; positive regulation of activated T cell proliferation; positive regulation of isotype switching to IgE isotypes; positive regulation of interleukin-10 production; B cell costimulation; positive regulation of MHC class II biosynthetic process; positive regulation of cell proliferation; positive regulation of B cell proliferation; positive regulation of T cell proliferation; positive regulation of interleukin-13 production; negative regulation of macrophage activation; cholesterol metabolic process; regulation of immune response; negative regulation of chronic inflammatory response; B cell activation; negative regulation of nitric oxide biosynthetic process; negative regulation of acute inflammatory response; positive regulation of mast cell degranulation; defense response to protozoan; T-helper 1 cell lineage commitment; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of immunoglobulin production; positive regulation of chemokine biosynthetic process; positive regulation of tyrosine phosphorylation of Stat5 protein; innate immune response in mucosa; positive regulation of T cell differentiation; positive regulation of B cell activation; regulation of inflammatory response; immune response; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription factor activity; positive regulation of protein amino acid phosphorylation; T-helper 2 cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of defense response to virus by host; negative regulation of T cell activation