Rabbit anti-Human, Rat Stat5a Polyclonal Antibody | anti-Stat5a antibody

Stat5a (S420) polyclonal antibody

Cat. Num. AAA3014583

• Gene Names: STAT5A; MGF; STAT5
• Reactivity: Human, Rat
• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Synonyms: Stat5a; Polyclonal Antibody; Stat5a (S420) polyclonal antibody; Signal transducer and activator of transcription 5A; STAT5A; STAT5; anti-Stat5a antibody

• Host: Rabbit
• Reactivity: Human, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Stat5a (S420) polyclonal antibody detects endogenous levels of Stat5a protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. The purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

• Applicable Applications for anti-Stat5a antibody: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
• Application Notes: WB: 1:500~1000; IHC: 1:50~1:200; IF: 1:50~1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 400-450 of Human Stat5a.
• Preparation and Storage: Store at 4 degree C short term. Aliquot and store at -20 degree C long term. Avoid freeze-thaw cycles.

Western Blot (WB)

(Western blot (WB) analysis of STAT5A pAb at 1:500 dilutionLane1:SP2/0 whole cell lysate(40ug)Lane2:SHSY5Y whole cell lysate(40ug)Lane3:MCF-7 whole cell lysate(40ug))

Related Product Information for anti-Stat5a antibody

Stat 5a protein is a transcription factor activated by hormone and cytokines. Two highly related, but distinct Stat 5 genes (Stat 5a and Stat 5b) were identified in mouse. The amino acid sequences of Stat 5a and Stat 5b show 96% sequence similarity, and both proteins are co expressed in most tissues of both virgin and lactating mice. However, differential accumulation of Stat 5a and Stat 5b mRNA has been reported for both muscle and mammary tissue. Stat 5a is critically involved in a variety of physiological functions, including reproduction, lactation, immune function and somatic growth.

NCBI and Uniprot Product Information

• NCBI GI #: 21618342
• NCBI GeneID: 6776
• NCBI Accession #: NP_003143.2
• NCBI GenBank Nucleotide #: NM_003152.3
• UniProt Accession #: P42229
• Molecular Weight: 90,647 Da
• NCBI Official Full Name: signal transducer and activator of transcription 5A
• NCBI Official Synonym Full Names: signal transducer and activator of transcription 5A
• NCBI Official Symbol: STAT5A
• NCBI Official Synonym Symbols: MGF; STAT5
• NCBI Protein Information: signal transducer and activator of transcription 5A
• UniProt Protein Name: Signal transducer and activator of transcription 5A
• Protein Family: Signal transducer and activator of transcription
• UniProt Gene Name: STAT5A
• UniProt Synonym Gene Names: STAT5
• UniProt Entry Name: STA5A_HUMAN

NCBI Description

The protein encoded by this gene is a member of the STAT family of transcription factors. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. This protein is activated by, and mediates the responses of many cell ligands, such as IL2, IL3, IL7 GM-CSF, erythropoietin, thrombopoietin, and different growth hormones. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 gene fusion is independent of cell stimulus and has been shown to be essential for the tumorigenesis. The mouse counterpart of this gene is found to induce the expression of BCL2L1/BCL-X(L), which suggests the antiapoptotic function of this gene in cells. [provided by RefSeq, Jul 2008]

Uniprot Description

STAT5A: transcription factor of the STAT family. Phosphorylated and activated by receptor-associated kinases downstream of many cytokines and growth-factor receptors. Activation of this protein in myeloma and lymphoma associated with a TEL/JAK2 fusion protein is essential for the tumorigenesis. Induces the expression of BCL2L1/BCL-X(L) in the mouse, suggesting an antiapoptotic function of this protein. Forms homo- or heterodimers that translocate into the nucleus where they regulate transcription. Two alternatively spliced isoforms have been described.

Protein type: Motility/polarity/chemotaxis; Oncoprotein; Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: nucleoplasm; cytosol

Molecular Function: signal transducer activity; protein binding; transcription factor activity

Biological Process: lactation; succinate metabolic process; peptidyl-tyrosine phosphorylation; oxaloacetate metabolic process; positive regulation of interleukin-2 biosynthetic process; T cell differentiation in the thymus; female pregnancy; positive regulation of multicellular organism growth; fatty acid metabolic process; positive regulation of mitotic cell cycle; positive regulation of activated T cell proliferation; 2-oxoglutarate metabolic process; positive regulation of natural killer cell differentiation; sequestering of lipid; negative regulation of mast cell apoptosis; natural killer cell differentiation; allantoin metabolic process; luteinization; development of secondary male sexual characteristics; regulation of steroid metabolic process; creatinine metabolic process; T cell homeostasis; positive regulation of gamma-delta T cell differentiation; Peyer's patch development; isoleucine metabolic process; negative regulation of erythrocyte differentiation; transcription, DNA-dependent; valine metabolic process; citrate metabolic process; regulation of multicellular organism growth; development of secondary female sexual characteristics; JAK-STAT cascade; positive regulation of natural killer cell mediated cytotoxicity; creatine metabolic process; regulation of transcription from RNA polymerase II promoter; regulation of epithelial cell differentiation; positive regulation of B cell differentiation; positive regulation of transcription from RNA polymerase II promoter; taurine metabolic process; positive regulation of inflammatory response