Immunohistochemistry (IHC)
(MBS9608883 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Rabbit anti-Human, Mouse IL-32 Polyclonal Antibody | anti-IL-32 antibody
IL-32 Antibody
Gene Names
IL32; NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
IL-32; Polyclonal Antibody; IL-32 Antibody; IL 32alpha; IL 32beta; IL 32delta; IL 32gamma; IL32; IL32_HUMAN; Interleukin 32; Interleukin 32 small; Interleukin 32 theta; Interleukin-32; interleukin-32 eta; Natural killer cell transcript 4; Natural killer cells protein 4; NK 4; NK4; OTTHUMP00000236040; OTTHUMP00000236041; OTTHUMP00000236042; OTTHUMP00000236043; OTTHUMP00000236044; OTTHUMP00000236045; OTTHUMP00000236046; OTTHUMP00000236047; OTTHUMP00000236048; OTTHUMP00000236049; OTTHUMP00000236050; OTTHUMP00000236051; OTTHUMP00000236052; OTTHUMP00000236053; OTTHUMP00000236054; OTTHUMP00000241545; OTTHUMP00000241602; OTTHUMP00000241603; OTTHUMP00000241604; OTTHUMP00000241605; OTTHUMP00000241606; OTTHUMP00000241607; OTTHUMP00000241608; OTTHUMP00000241609; OTTHUMP00000241610; OTTHUMP00000241611; OTTHUMP00000241612; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; Tumor necrosis factor alpha-inducing factor; anti-IL-32 antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
IL-32 antibody detects endogenous levels of total IL-32
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
188
Applicable Applications for anti-IL-32 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:1000-3000
IHC: 1:200
IHC: 1:200
Immunogen
A synthesized peptide derived from human IL-32
Subcellular Location
Secreted.
Tissue Specificity
Selectively expressed in lymphocytes. Expression is more prominent in immune cells than in non-immune cells.
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Immunohistochemistry (IHC)
(MBS9608883 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9608883 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Western Blot (WB)
(Western blot analysis IL-32 using K562 whole cell lysates)
Western Blot (WB)
(Western blot analysis IL-32 using K562 whole cell lysates)
Related Product Information for anti-IL-32 antibody
Function: Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 26 kDa
Predicted: 27 kDa
Predicted: 27 kDa
NCBI Official Full Name
interleukin-32 isoform B
NCBI Official Synonym Full Names
interleukin 32
NCBI Official Symbol
IL32
NCBI Official Synonym Symbols
NK4; TAIF; TAIFa; TAIFb; TAIFc; TAIFd; IL-32beta; IL-32alpha; IL-32delta; IL-32gamma
NCBI Protein Information
interleukin-32
UniProt Protein Name
Interleukin-32
UniProt Gene Name
IL32
UniProt Synonym Gene Names
NK4; TAIF; IL-32
NCBI Description
This gene encodes a member of the cytokine family. The protein contains a tyrosine sulfation site, 3 potential N-myristoylation sites, multiple putative phosphorylation sites, and an RGD cell-attachment sequence. Expression of this protein is increased after the activation of T-cells by mitogens or the activation of NK cells by IL-2. This protein induces the production of TNFalpha from macrophage cells. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
Uniprot Description
Cytokine that may play a role in innate and adaptive immune responses. It induces various cytokines such as TNFA/TNF-alpha and IL8. It activates typical cytokine signal pathways of NF-kappa-B and p38 MAPK.
Research Articles on IL-32
Similar Products
Product Notes
The IL-32 il32 (Catalog #AAA9608883) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The IL-32 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's IL-32 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:1000-3000 IHC: 1:200. Researchers should empirically determine the suitability of the IL-32 il32 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "IL-32, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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